Figure 6From: MITEs in the promoters of effector genes allow prediction of novel virulence genes in Fusarium oxysporumDeletion of the mimp in the shared promoter region of SIX3 and SIX5 does not affect expression of the two genes. (A) Schematic representation of the SIX3/SIX5 locus. Black lines: deleted promoter fragments (length of the deletion in bp; pink box: mimp; yellow arrow: SIX gene; orange circles: sequence matching AAGTCGGCAGTT[AG] motif, which is enriched in SIX1-7 promoters. (B) In vitro expression of SIX3 and SIX5 in the promoter deletion strains. Mycelium of the indicated Fol strains was collected after growth in minimal medium, RNA was extracted and RT-PCR was performed. (C) In planta expression of SIX3 and SIX5 in the promoter deletion strains. Roots of ten days old susceptible (without resistance genes) or resistant (encoding the I-2 resistance protein that recognizes Six3) tomato seedlings were inoculated with the indicated Fol strains or with water (mock). Roots were harvested 9 dpi (days post inoculation), RNA was extracted and RT-PCR was performed as described above. Expression of SIX1 was used as a control. (D) Disease assay of tomato plants. Performed as above. Error bars indicate the 95% confidence interval of the mean. Numbers indicate the strains with which the tomato plants were inoculated: (1) mock, (2) Fol007 (WT), (3) SIX3p539#2, (4) SIX3p807#1, (5) SIX3p807#2, (6) SIX3p859#1, (7) SIX3p859#2. A black box marks interactions where recognition of Six3 by I-2 is broken or where no disease is caused.Back to article page