From: Identification and functional analysis of cytochrome P450 complement in Streptomyces virginiaeIBL14
Strains | Relevant properties | Source |
---|---|---|
Escherichia coli | ||
JM109 | Cloning host, genotype:endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, (lac-proAB), [F’ traD36, proAB, laqIqZΔM15] | Promega |
JM109 (DE3) | Expression host, genotype:endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, λ–, Δ(lac-proAB), [F’, traD36, proAB, lacIqZΔM15], lDE3 | Promega |
Bacillus subtilis | ||
WB800N | Secretion host with resistance to neomycin, genotype: nprE aprE epr bpr mpr :: ble nprB :: bsr vpr wprA :: hyg cm :: neo; NeoR | Mo Bi Tec |
Streptomyces virginiae | ||
IBL14 | Wild type | Our lab |
Plasmids | ||
pET22b | Expression vector in E. coli | Novagen |
pHCMC05 | Shuttle plasmid | BGSC |
pET22b-svh01-svf09 | The fragment of svh01 and svf09 were digested with NdeI/EcoRIand EcoRI/Hind Ш, respectively, and ligated into the NdeIand Hind Ш sites of pET22b | This study |
pHCMC05-svu022 | The gene of svu022 digested with BamHI/SmaIligated into BamHI/SmaIdigested pHCMC05 | This study |