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Table 5 Microorganisms and plasmids used in this study

From: Identification and functional analysis of cytochrome P450 complement in Streptomyces virginiaeIBL14

Strains

Relevant properties

Source

Escherichia coli

JM109

Cloning host, genotype:endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, (lac-proAB), [F’ traD36, proAB, laqIqZΔM15]

Promega

JM109 (DE3)

Expression host, genotype:endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, λ–, Δ(lac-proAB), [F’, traD36, proAB, lacIqZΔM15], lDE3

Promega

Bacillus subtilis

WB800N

Secretion host with resistance to neomycin, genotype: nprE aprE epr bpr mpr :: ble nprB :: bsr vpr wprA :: hyg cm :: neo; NeoR

Mo Bi Tec

Streptomyces virginiae

IBL14

Wild type

Our lab

Plasmids

pET22b

Expression vector in E. coli

Novagen

pHCMC05

Shuttle plasmid

BGSC

pET22b-svh01-svf09

The fragment of svh01 and svf09 were digested with NdeI/EcoRIand EcoRI/Hind Ш, respectively, and ligated into the NdeIand Hind Ш sites of pET22b

This study

pHCMC05-svu022

The gene of svu022 digested with BamHI/SmaIligated into BamHI/SmaIdigested pHCMC05

This study