Skip to main content

Table 6 The PCR primers used in this study

From: Identification and functional analysis of cytochrome P450 complement in Streptomyces virginiaeIBL14

Primer

Primer sequencea(5′to3′)

Restriction site

pSVH01F

GCCCCCCATATGAGTGAGTCCCTCCACACCGTC

NdeI

pSVH01R

GGAGGAATTCACTTCGCGTCCCAGGTG

EcoRI

pSVF09F

CCGGAATTCGGGACGCGAAGTGAGCGCGG

EcoRI

pSVF09R

CCCAAGCTTTCAGGCGGAGGGTGGGCGG

Hind III

pSVU022F

CTGGATCCATGAGCTGCCCGATCGACC

BamHI

pSVU022R

CCTAAGCTTTCAGGGGTGCAGGCGTACCG

SmaI

  1. aThe underlined sequence are recognition sites of restriction enzymes and the nucleotides before it are the protected bases. All primers are designed by Primer Premier 5.0 and verified by Oligo 7.0.
Back to article page

BMC Genomics

ISSN: 1471-2164

Contact us