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Table 1 Summary of the annotation of the secondary Amblyomma americanum EST assembly

From: Why so many unknown genes? Partitioning orphans from a representative transcriptome of the lone star tick Amblyomma americanum

A. UniProtKB Annotation Number
Input sequences 14,310
Short (< 33 amino acids) returns 171
Weak (e-value > 1E-5) returns 145
Total unannotated returns 10,192
Annotated singletons 2,875
Annotated contigs 1,243
Total annotated sequences 4,118
B. Peptide Database: Species No. matches
Ixodes scapularis 2,842
Tetranychus urticae 143
Daphnia pulex 208
Pediculus humanus 107
Acyrthosiphon pisum 225
Apis mellifera 140
Tribolium castaneum 218
Bombyx mori 28
Aedes aegypti 49
Anopheles gambiae 39
Culex quinquefasciatus 46
Drosophila melanogaster 52
Total Hits 4,099 (28.6%)
  1. Statistics given for annotation against (A) the UniProtKB protein database and (B) a collection of arthropod datasets. A BLAST search of A. americanum ESTs against a compilation of 12 arthropod peptide datasets was conducted. Species are arranged to reflect increasing phylogenetic distance from A. americanum.