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Table 1 Summary of the annotation of the secondary Amblyomma americanum EST assembly

From: Why so many unknown genes? Partitioning orphans from a representative transcriptome of the lone star tick Amblyomma americanum

A. UniProtKB Annotation

Number

Input sequences

14,310

Short (< 33 amino acids) returns

171

Weak (e-value > 1E-5) returns

145

Total unannotated returns

10,192

Annotated singletons

2,875

Annotated contigs

1,243

Total annotated sequences

4,118

B. Peptide Database: Species

No. matches

Ixodes scapularis

2,842

Tetranychus urticae

143

Daphnia pulex

208

Pediculus humanus

107

Acyrthosiphon pisum

225

Apis mellifera

140

Tribolium castaneum

218

Bombyx mori

28

Aedes aegypti

49

Anopheles gambiae

39

Culex quinquefasciatus

46

Drosophila melanogaster

52

Total Hits

4,099 (28.6%)

  1. Statistics given for annotation against (A) the UniProtKB protein database and (B) a collection of arthropod datasets. A BLAST search of A. americanum ESTs against a compilation of 12 arthropod peptide datasets was conducted. Species are arranged to reflect increasing phylogenetic distance from A. americanum.