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Table 2 Comparison of quality measures for annotated and unannotated ESTs

From: Why so many unknown genes? Partitioning orphans from a representative transcriptome of the lone star tick Amblyomma americanum

  

Annotated

Unannotated

 Mean ORF length (nts)

Sequences

339.9

183.4

 

Contigs

376.7

213.1

   Mean length (nts)

Sequences

594.9

536.0

 

Contigs

746.5

735.8

Number w/o start codon

Sequences

0

398

 

Contigs

0

38

   Mean GC-content

Sequences

50

45.2

 

Contigs

49.7

45.1

  1. Comparisons are included for mean open-reading frame (ORF) length, mean nucleotide length, number of ESTs lacking a start codon, and mean GC-content. Statistics were obtained using OrfPredictor (ORF length and start codons) and Geneious software (nucleotide length and GC-content).
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BMC Genomics

ISSN: 1471-2164

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