Figure 4
Metabolic profiling of Cg>RNAidHHEX-V15721, Dcr-2. A. Wandering third-instar females of Cg>RNAidHHEX-V15721, Dcr-2 are hyperglycemic. Each measurement is made from a pooled sample of hemolymph from 5–8 animals; dark red line segments show mean values. p < 0.029. B–C. Wandering third-instar and newly eclosed adult females of Cg>RNAidHHEX-V15721, Dcr-2 have reduced body size. Each measurement is the mean per-animal mass of a group of 6–10 animals; dark red line segments show mean values. p < 0.001 for both larvae and adults. D–E. Wandering third-instar and newly eclosed adult females of Cg>RNAidHHEX-V15721, Dcr-2 have reduced triglyceride levels. Each measurement is made from a pooled sample of whole-animal homogenate from 6–10 animals; dark red line segments show mean values. p < 0.007 for both larvae and adults (p < 0.002 for adults when one extreme outlier is excluded). All p-values for metabolic data were calculated using a bidirectional t-test without assuming equal variances. More complete details are in Additional file 1: Tables S6–S8.