Skip to main content

Table 2 Characteristics of the Douglas-fir transcriptome assembly using Newbler v2.3

From: A SNP resource for Douglas-fir: de novotranscriptome assembly and SNP detection and validation

  

Length (nt)

Statistic

Number

Mean

Median

N50

Total

Reads used by Newbler*

2,764,549

360

392

416

996,614,802

Reads assembled by Newbler†

2,544,087

364

394

416

925,577,338

Isotigs§

38,589

1390

1141

1883

53,622,767

Isogroups

25,002

1443

1181

1864

36,069,331

 Isogroups with 1 isotig (I1)

18,774

1334

1053

1750

25,046,862

 Isogroups with >1 isotig (IM)‡

6228

1770

1547

2141

11,022,469

Singletons

102,623

356

384

413

36,504,221

Total (isogroups + singletons)

127,625

569

413

517

72,573,552

  1. * The input number of reads is less than the total in Table 1 (2.96 × 106) because reads shorter than 50 nt were excluded. Statistics were calculated using the sequences actually used in the assembly after applying a default minimum length of 40 for reads trimmed by Newbler.
  2. † Includes reads that assembled as complete reads or as partial reads.
  3. § Isotigs ≥ 200 nt were deposited at DDBJ/EMBL/GenBank under accession GAEK01000000.
  4. ‡ Statistics for the IM isogroups were calculated using the longest isotig in each isogroup.