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Table 3 Comparison between Douglas-fir isotigs and white spruce unigenes[16]

From: A SNP resource for Douglas-fir: de novotranscriptome assembly and SNP detection and validation

      Number of isotigs  
Class* No. of WS matches Do other DF match the same WS?§ Do other matching DF overlap? Isotig confidence# I1 subset (1 isotig per isogroup) (18,774) IM subset (>1 isotig per isogroup) (19,815) Example visual representations@
C1 1 No - Highest 5140 261
C2 2+ No - Higher 896 88
C3 1 Yes No Higher 1767 577
C4 2+ Yes No Medium 586 159
C5 1 Yes Yes Lower 1736 6974
C6 2+ Yes Yes Lowest 3405 7040
  Subtotal - - - 13,530 15,099  
C7 No matches - - Unknown 5244 4716  
  1. *Douglas-fir (DF) isotigs were categorized into seven classes (C1-C7) and three levels of confidence based on their relationships to white spruce (WS) contigs using the SCARF program [68].
  2. Number of white spruce contigs that matched the Douglas-fir query.
  3. §‘Yes’ indicates that at least one non-query isotig also matched the same white spruce contig.
  4. ‘Yes’ indicates that the query and at least one non-query isotig matched the same region of the white spruce contig (overlapped).
  5. #Subjective level of confidence in the isotig assembly based on the information presented in columns 2–4.
  6. @Cross-hatched bars represent white spruce contigs, black bars represent query Douglas-fir isotigs, and white bars represent non-query Douglas-fir isotigs.