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Table 4 Numbers and percentages of Douglas-fir sequences with matches to sequences in three protein databases*

From: A SNP resource for Douglas-fir: de novotranscriptome assembly and SNP detection and validation

 

Isogroups (25,002)†

Singletons (102,623)§

 

Isogroups with 1 isotig (I1 = 18,774)

Isogroups with >1 isotig (IM = 6228)

Singletons (S = 102,623)

Database

Number

Percent

Number

Percent

Number

Percent

Uniref50

15,054

80.2

3446

55.3

25,757

25.1

TAIR10

13,749

73.2

3260

52.3

15,907

15.5

Annot8r

11,733

62.5

2862

46.0

14,836

14.5

  1. *Matches were recorded for isogroups and singletons at a tBLASTX E-value < 10-5.
  2. †Isogroups are Newbler v2.3 isogroups. For the isogroups with more than 1 isotig (IM subset), a hit was counted only if all isotigs matched the same protein in the database.
  3. §Singletons are 454 reads that did not assemble with any other reads.