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Table 4 Numbers and percentages of Douglas-fir sequences with matches to sequences in three protein databases*

From: A SNP resource for Douglas-fir: de novotranscriptome assembly and SNP detection and validation

  Isogroups (25,002) Singletons (102,623)§
  Isogroups with 1 isotig (I1 = 18,774) Isogroups with >1 isotig (IM = 6228) Singletons (S = 102,623)
Database Number Percent Number Percent Number Percent
Uniref50 15,054 80.2 3446 55.3 25,757 25.1
TAIR10 13,749 73.2 3260 52.3 15,907 15.5
Annot8r 11,733 62.5 2862 46.0 14,836 14.5
  1. *Matches were recorded for isogroups and singletons at a tBLASTX E-value < 10-5.
  2. Isogroups are Newbler v2.3 isogroups. For the isogroups with more than 1 isotig (IM subset), a hit was counted only if all isotigs matched the same protein in the database.
  3. §Singletons are 454 reads that did not assemble with any other reads.