MicroRNAs and their putative targets in Brassica napusseed maturation

BMC Genomics

Table 1 Sequencing statistics

Sequencing platform	Library	Total reads	Clipped*	Cleaned**
SOLiD dataset	flower buds	12,801,066	11,282,118	11,282,091
	10d	13,654,854	11,230,801	11,230,779
	15d	8,183,585	6,823,454	6,823,440
	20d	7,454,670	6,194,847	6,194,827
	25d	9,243,505	8,271,307	8,271,295
	30d	7,130,691	6,074,794	6,074,781
	35d	6,436,710	5,370,745	5,370,732
	40d	6,944,646	5,789,097	5,789,076
	45d	7,705,418	6,954,348	6,954,334
	50d	8,749,906	6,829,547	6,829,528
	Subtotal	88,305,051	74,821,058	74,820,883
Illumina dataset	15DEm	14,034,094	10,378,052	10,371,178
	15DEndo	24,739,789	19,000,135	18,916,066
	15DSC	17,442,131	14,601,621	14,594,202
	25 DC	15,168,491	10,832,772	10,831,009
	25DEm	11,925,734	10,937,990	10,936,745
	25DEndo	15,610,323	13,157,907	13,155,132
	25DH	15,885,961	13,183,381	13,180,455
	25DR	15,496,019	13,958,721	13,955,227
	25DSC	12,782,271	11,308,036	11,306,170
	35DEm	16,913,533	15,482,754	15,476,635
	35DEndo	17,644,795	10,178,579	10,173,946
	35DSC	16,381,235	11,488,796	11,483,795
	45 DC	15,997,842	13,743,381	13,633,211
	45DEm	17,405,343	13,556,990	13,543,113
	45DH	25,222,060	20,437,149	20,369,083
	45DR	24,660,758	22,099,699	22,092,547
	45DSC	17,770,899	10,608,884	10,603,168
	Subtotal	295,081,278	234,954,847	234,621,682
Total		383,386,329	309,775,905	309,442,565

Notes:
1) *Discarded (clipped) Illumina Adaptor: ATCTCGTATGCCGTCTTCTGCTTG (length < 19nt, adaptor & Ns only reads).
2) *Clipped Solid Adaptor: CGCCTTGGCCGTACAGCAG (length < 19nt, adaptor & Ns only reads).
3) **Cleaned single nucleotide repeats (poly As, Cs, Ts, Gs).

ISSN: 1471-2164