Table 2 The most frequently sequenced miRNA/variants in B. napus seeds (>3000 total reads)
From: MicroRNAs and their putative targets in Brassica napusseed maturation
miRNA family | miRNA sequence | Given miRNA name | Identical sequence (Mature) | Homologous MIRNA (Precursor) | Total reads |
|---|---|---|---|---|---|
miR156 | TGACAGAAGAGAGTGAGCAC | bna-miR156_v1 | ath-miR156a | ath-MIR156a | 3282238 |
| Â | TGACAGAAGAGAGTAAGCAC | bna-miR156_v2 | Â | ath-MIR156a | 187170 |
| Â | TGACAGAAGAGAGTGAACAC | bna-miR156_v3 | Â | ath-MIR156c | 71366 |
| Â | TTGACAGAAGAAAGAGAGCAC | bna-miR156_v4 | smo-miR156c | smo-MIR156c | 66413 |
| Â | TGACAGAAGAGAGTGAGCACA | bna-miR156_v5 | bna-miR156a | bna-MIR156c | 39023 |
| Â | TGACAGAAGAGAGTCAGCAC | bna-miR156_v6 | Â | osa-MIR156c | 38941 |
| Â | TGACAGAAGAGAGGGAGCAC | bna-miR156_v7 | ptc-miR156k | ptc-MIR156k | 18907 |
| Â | TGACAGAAGAGAGTGAGCAA | bna-miR156_v8 | Â | osa-MIR156e | 15166 |
| Â | TGACAGAAGAGAGTGAGCAT | bna-miR156_v9 | Â | ath-MIR156c | 14021 |
| Â | TGACAGAAGAGAGTTAGCAC | bna-miR156_v10 | Â | ssp-MIR156 | 13172 |
| Â | TGACAGAAGAGAGTGAGAAC | bna-miR156_v11 | Â | ath-MIR156a | 10071 |
| Â | TGACAGAAGAGAGTGAGCACC | bna-miR156_v12 | Â | bdi-MIR156c | 9628 |
| Â | TTGACAGAAGAGAGTGAGCAC | bna-miR156_v13 | gma-miR156k | gma-MIR156k | 9212 |
| Â | TGCCAGAAGAGAGTGAGCAC | bna-miR156_v14 | Â | gma-MIR156h | 8191 |
| Â | TGACAGAAGAGAGCGAGCAC | bna-miR156_v15 | zma-miR156k | zma-MIR156k | 7663 |
| Â | CGACAGAAGAGAGTGAGCAC | bna-miR156_v16 | ath-miR156g | ath-MIR156g | 6529 |
| Â | TGACAGAAGAAAGAGAGCAC | bna-miR156_v17 | ath-miR156h | ath-MIR156h | 5933 |
| Â | TGACAGAAGAGAGGGAGCAA | bna-miR156_v18 | Â | vvi-MIR156a | 5778 |
| Â | TTGACAGAAGAGAGCGAGCAC | bna-miR156_v19 | Â | sbi-MIR156e | 5582 |
| Â | GCTCACTGCTTTATCTGTCAGA | bna-miR156_v20 | Â | aly-MIR156c | 5492 |
| Â | TGACAGAAGAGAGTGGGCAC | bna-miR156_v21 | Â | zma-MIR156i | 5326 |
| Â | GCTCACTGCTCTTTCTGTCAGA | bna-miR156_v22 | aly-miR156a* | aly-MIR156a | 5242 |
| Â | TGACAGAAGAGAGTGAGCGC | bna-miR156_v23 | Â | osa-MIR156b | 5198 |
| Â | TGACAGAAGAGAGTGTGCAC | bna-miR156_v24 | Â | osa-MIR156i | 4981 |
| Â | TGACAGAAGAGAGTGAGCCC | bna-miR156_v25 | Â | ctr-MIR156 | 4782 |
| Â | TTGACAGAAGAGACCGAGCAC | bna-miR156_v26 | Â | zma-MIR156k | 3734 |
| Â | TTGACAGAAGAAAGAAAGCAC | bna-miR156_v27 | Â | ath-MIR156h | 3729 |
| Â | TGACAGAAGAGAGTGAGCACT | bna-miR156_v28 | Â | vvi-MIR156e | 3661 |
| Â | TGACAGAAGAGAGTGCGCAC | bna-miR156_v29 | Â | ath-MIR156f | 3555 |
| Â | TGACATAAGAGAGTGAGCAC | bna-miR156_v30 | Â | ath-MIR156a | 3082 |
| Â | TGACAGAAGAGAGTGACCAC | bna-miR156_v31 | Â | zma-MIR156i | 3043 |
miR157 | TTGACAGAAGATAGAGAGCAC | bna-miR157_v1 | ath-miR157a | ath-MIR157c | 9274 |
miR158 | TTTCCAAATGTAGACAAAGCA | bna-miR158_v1 | Â | ath-MIR158a | 379019 |
| Â | TTTCCAAATGTAGACAAAGC | bna-miR158_v2 | Â | aly-MIR158a | 35353 |
| Â | TCCCAAATGTAGACAAAGCA | bna-miR158_v3 | ath-miR158a | ath-MIR158a | 18654 |
| Â | TTCCAAATGTAGACAAAGCA | bna-miR158_v4 | Â | ath-MIR158a | 8078 |
miR159 | TTTGGATTGAAGGGAGCTCTA | bna-miR159_v1 | ath-miR159a | ath-MIR159a | 1270354 |
| Â | TTTGGATTGAAGGGAGCTCT | bna-miR159_v2 | Â | bra-MIR159a | 51891 |
| Â | TTTGGATTGAAGGGAACTCTA | bna-miR159_v3 | Â | csi-MIR159 | 46735 |
| Â | TTTGGATTGAAGGGAGCCCTA | bna-miR159_v4 | Â | bna-MIR159 | 8153 |
| Â | TTTGGATTGAAGGGACCTCTA | bna-miR159_v5 | Â | ptc-MIR159a | 7927 |
| Â | TTTGTATTGAAGGGAGCTCTA | bna-miR159_v6 | Â | bna-MIR159 | 6853 |
| Â | TTTGGATTGAAGGGAGATCTA | bna-miR159_v7 | Â | ptc-MIR159a | 3935 |
| Â | CTTGCATATCTTAGGAGCTTT | bna-miR159_v8 | Â | ptc-MIR159c | 3806 |
| Â | TTTGGATTGAAGGGAGCACTA | bna-miR159_v9 | Â | bna-MIR159 | 3448 |
miR160 | TGCCTGGCTCCCTGTATGCCA | bna-miR160_v1 | ath-miR160a | ath-MIR160a | 91013 |
| Â | TGCCTGGCTCCCTGTATACCA | bna-miR160_v2 | Â | zma-MIR160e | 3993 |
miR161 | TCAATGCACTGAAAGTGACTA | bna-miR161_v1 | bna-miR161 | bna-MIR161 | 5423 |
miR162 | TCGATAAACCTCTGCATCCAG | bna-miR162_v1 | ath-miR162a | ath-MIR162a | 15091 |
miR164 | TGGAGAAGCAGGGCACGTGCA | bna-miR164_v1 | ath-miR164a | ath-MIR164a | 4298 |
miR165 | TCGGACCAGGCTTCATCCCCC | bna-miR165_v1 | ath-miR165a | ath-MIR165a | 49308 |
| Â | TCGGACCAGGCTTCATCCCC | bna-miR165_v2 | aly-miR165a | aly-MIR165a | 8234 |
miR166 | TCGGACCAGGCTTCATTCCCC | bna-miR166_v1 | ath-miR166a | ath-MIR166a | 149247 |
| Â | GGAATGTTGTCTGGCTCGAGG | bna-miR166_v2 | zma-miR166c* | zma-MIR166c | 7006 |
| Â | TCGGACCAGGCTTCATACCCC | bna-miR166_v3 | Â | zma-MIR166d | 3864 |
| Â | GTCGGACCAGGCTTCATTCCC | bna-miR166_v4 | Â | aly-MIR166a | 3710 |
| Â | TCGGACCAGGCTTCATTCCC | bna-miR166_v5 | zma-miR166h | zma-MIR166h | 3039 |
| Â | TGAAGCTGCCAGCATGATCTA | bna-miR167_v1 | ath-miR167a | ath-MIR167a | 436575 |
miR167 | TGAAGCTGCCAGCATAATCTA | bna-miR167_v2 | Â | gma-MIR167d | 28902 |
| Â | TGAAGCTGCCAGCATGACCTA | bna-miR167_v3 | Â | bdi-MIR167a | 18620 |
| Â | GATCATGTTCGCAGTTTCACC | bna-miR167_v4 | aly-miR167a* | aly-MIR167a | 13484 |
| Â | TGAAGCTGCCAGCATGATCT | bna-miR167_v5 | Â | gma-MIR167g | 10622 |
| Â | TGAAGCTGCCAGCATCATCTA | bna-miR167_v6 | Â | bna-MIR167b | 4726 |
| Â | TGAAGCTGCCAGCATGAACTA | bna-miR167_v7 | Â | mtr-MIR167 | 4258 |
| Â | GAAGCTGCCAGCATGATCTA | bna-miR167_v8 | Â | zma-MIR167c | 4011 |
miR168 | TCGCTTGGTGCAGGTCGGGAC | bna-miR168_v1 | Â | bdi-MIR168 | 5401 |
| Â | TCGCTTGGTGCAGGTCGGGAA | bna-miR168_v2 | ath-miR168a | ath-MIR168a | 5113 |
miR169 | CAGCCAAGGATGACTTGCCGA | bna-miR169_v1 | ath-miR169a | ath-MIR169a | 10010 |
miR171 | TGATTGAGCCGCGCCAATATC | bna-miR171_v1 | ath-miR171a | ath-MIR171a | 54482 |
| Â | TGATTGAGCCGCGCCAATACC | bna-miR171_v2 | Â | sly-MIR171c | 33222 |
| Â | TGATTGAGCCGCGTCAATATC | bna-miR171_v3 | mtr-miR171b | mtr-MIR171b | 16452 |
| Â | TGATTGAGCCGCGTCAATACC | bna-miR171_v4 | Â | mtr-MIR171b | 5107 |
miR172 | AGAATCTTGATGATGCTGCAG | bna-miR172_v1 | ath-miR172c | ath-MIR172c | 422468 |
| Â | GGAATCTTGATGATGCTGCAT | bna-miR172_v2 | ath-miR172e | ath-MIR172e | 9098 |
| Â | AGAATCTTGATGATGATGCAG | bna-miR172_v3 | Â | aly-MIR172d | 8479 |
| Â | AGAATCTTGATGATGCTGCAT | bna-miR172_v4 | ath-miR172a | ath-MIR172a | 6876 |
| Â | AGAATCTTGATGATGTTGCAG | bna-miR172_v5 | Â | ath-MIR172c | 4545 |
| Â | AGAATCTTGATGATGCTGCA | bna-miR172_v6 | zma-miR172a | zma-MIR172a | 3850 |
miR319 | TTGGACTGAAGGGAGCTCCCT | bna-miR319_v1 | ath-miR319a | ath-MIR319a | 46441 |
| Â | TTGGACTGAAGGGAGCTCCC | bna-miR319_v2 | mtr-miR319 | mtr-MIR319 | 37491 |
| Â | TTGGACTGAAGGGAACTCCCT | bna-miR319_v3 | Â | vvi-MIR319f | 12179 |
| Â | TTGGACTGAAGGGAGCTCCTT | bna-miR319_v4 | ath-miR319c | ath-MIR319c | 6369 |
| Â | TTGGACTGAAGGGAACTCCC | bna-miR319_v5 | Â | ptc-MIR319b | 6132 |
| Â | TTTGGACTGAAGGGAGCTCCT | bna-miR319_v6 | vvi-miR319e | vvi-MIR319e | 3347 |
miR390 | AAGCTCAGGAGGGATAGCGCC | bna-miR390_v1 | ath-miR390a | ath-MIR390a | 13211 |
| Â | CGCTGTCCATCCTGAGTTTC | bna-miR390_v2 | Â | bna-MIR390b | 9543 |
| Â | CGCTGTCCATCCTGAGTTTCA | bna-miR390_v3 | Â | bna-MIR390b | 3655 |
miR394 | TTGGCATTCTGTCCACCTCC | bna-miR394_v1 | ath-miR394a | ath-MIR394a | 19062 |
| Â | TTTGGCATTCTGTCCACCTCC | bna-miR394_v2 | Â | bdi-MIR394 | 3195 |
miR395 | CTGAAGTGTTTGGGGGAACTC | bna-miR395_v1 | ath-miR395a | ath-MIR395a | 31659 |
| Â | CTGAAGTGTTTGGGGAAACTC | bna-miR395_v2 | Â | osa-MIR395t | 4325 |
miR396 | TTCCACAGCTTTCTTGAACTT | bna-miR396_v1 | ath-miR396b | ath-MIR396b | 3623 |
miR397 | TCATTGAGTGCAGCGTTGATGT | bna-miR397_v1 | bna-miR397a | bna-MIR397a | 3500 |
miR400 | TATGAGAGTATTATAAGTCAC | bna-miR400_v1 | ath-miR400 | ath-MIR400 | 10737 |
miR403 | TTAGATTCACGCACAAACTCG | bna-miR403_v1 | ath-miR403 | ath-MIR403 | 36064 |
miR408 | ACAGGGAACAAGCAGAGCATG | bna-miR408_v1 | Â | aly-MIR408 | 18138 |
miR824 | TAGACCATTTGTGAGAAGGGA | bna-miR824_v1 | ath-miR824 | ath-MIR824 | 72249 |
| Â | TAGACCATTTGTGAGAAGGG | bna-miR824_v2 | Â | bra-MIR824 | 8270 |
| Â | TAGACCATTTGTGAGAAGGGAA | bna-miR824_v3 | Â | bna-MIR824 | 7041 |
| Â | TAGACCATTTGTGAGAAAGGA | bna-miR824_v4 | Â | bol-MIR824 | 4663 |
| Â | TAGACCATTTGTGAGTAGGGA | bna-miR824_v5 | Â | bra-MIR824 | 4604 |
| Â | TAGACCATTTGTGAGAAGAGA | bna-miR824_v6 | Â | bra-MIR824 | 4267 |
| Â | CCTTCTCATCGATGGTCTAGA | bna-miR824_v7 | aly-miR824* | aly-MIR824 | 4227 |
miR827 | TTAGATGACCATCAACAAATA | bna-miR827_v1 | Â | tcc-MIR827 | 59977 |
miR845 | CGGCTCTGATACCAATTGATG | bna-miR847_v1 | ath-miR845a | ath-MIR845a | 4297 |
miR858 | TTTCGTTGTCTGTTCGACCTT | bna-miR858_V1 | ath-miR858 | ath-MIR858 | 7316 |
| Â | TTCGTTGTCTGTTCGACCTTG | bna-miR858_V2 | ath-miR858b | ath-MIR858b | 5218 |
miR894 | CGTTTCACGTCGGGTTCACCA | bna-miR894_v1 | Â | ppt-MIR894 | 4474 |
miR916 | GGCCTATTAGCTCAGTTGGTTAG | bna-miR916_v1 | Â | cre-MIR916 | 7489 |
miR1885 | TACATCTTCTCCGCGGAAGCTC | bna-miR1885_v1 | Â | bra-MIR1885 | 13998 |