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Table 5 RT-PCR determination of gene expression in MAV, striatum, and parietal cortex

From: Comparison of the global gene expression of choroid plexus and meninges and associated vasculature under control conditions and after pronounced hyperthermia or amphetamine toxicity

  

3 hr

1 day

NCBI gene symbol

Brain Region

Control

AMPH

EIH

AMPH

EIH

Angpt2

MAV

1.08 ± 0.37

0.82 ± 0.23 (0.76)

0.81 ± 0.17 (0.75)

N.D.

N.D.

Parietal Cortex

0.02 ± 0.004

0.04 ± 0.01 (2.00)

0.03 ± 0.01 (1.50)

N.D.

N.D.

Striatum

0.03 ± 0.001

0.06 ± 0.02 (2.00)

0.04 ± 0.01 (1.33)

N.D.

N.D.

Aqp1

MAV

6.67 ± 1.61

5.30 ± 1.89 (0.79)

5.68 ± 1.11 (0.85)

N.D.

N.D.

Parietal Cortex

0.01 ± 0.003

0.08 ± 0.17 (8.00)

0.02 ± 0.02 (2.00)

N.D.

N.D.

Striatum

0.02 ± 0.003

0.04 ± 0.01 (2.00)

0.02 ± 0.003 (1.00)

N.D.

N.D.

Aqp4

MAVb,c

1.00 ± 0.34

1.11 ± 0.27 (1.11)

1.74 ± 0.11 (1.74)

N.D.

N.D.

Parietal Cortex

0.75 ± 0.03

0.73 ± 0.12 (0.97)

0.79 ± 0.10 (1.05)

N.D.

N.D.

Striatum

1.86 ± 0.01

2.14 ± 0.24 (1.15)

2.01 ± 0.36 (1.08)

N.D.

N.D.

Cldn5

MAVa

3.95 ± 1.18

0.99 ± 0.52 (0.25)

1.53 ± 0.88 (0.39)

N.D.

N.D.

Parietal Cortex

0.41 ± 0.06

0.19 ± 0.05 (0.46)

0.19 ± 0.12 (0.46)

N.D.

N.D.

Striatum

0.45 ± 0.04

0.20 ± 0.06 (0.44)

0.28 ± 0.13 (0.62)

N.D.

N.D.

Esam

MAV

3.55 ± 1.16

4.29 ± 1.19 (1.21)

2.68 ± 0.42 (0.75)

N.D.

N.D.

Parietal Cortex

0.25 ± 0.03

0.66 ± 0.14 (2.64)

0.68 ± 0.42 (2.72)

N.D.

N.D.

Striatum

0.35 ± 0.02

0.80 ± 0.21 (2.29)

0.55 ± 0.04 (1.57)

N.D.

N.D.

F11r

MAVa,b

1.63 ± 0.27

0.96 ± 0.15 (0.59)

0.96 ± 0.20 (0.59)

N.D.

N.D.

Parietal Cortex

0.11 ± 0.02

0.10 ± 0.01 (0.91)

0.10 ± 0.03 (0.91)

N.D.

N.D.

Striatum

0.19 ± 0.02

0.15 ± 0.01 (0.79)

0.16 ± 0.05 (0.84)

N.D.

N.D.

Fst

MAVa,d

0.11 ± 0.05

2.23 ± 1.33 (20.27)

0.33 ± 0.15 (3.00)

0.25 ± 0.16 (2.78)

0.09 ± 0.02

Parietal Cortex

0.11 ± 0.05

0.21 ± 0.06 (1.91)

0.22 ± 0.04 (2.00)

N.D.

N.D.

Striatum

0.19 ± 0.05

0.40 ± 0.20 (2.10)

0.33 ± 0.04 (1.74)

N.D.

N.D.

Has1

MAVa,d

0.05 ± 0.02

0.20 ± 0.08 (2.0)

0.760 ± 0.670 (15.20)

0.21 ± 0.07 (1.75)

0.12 ± 0.02

Parietal Cortex

0.04 ± 0.01

0.11 ± 0.03 (2.75)

0.13 ± 0.04 (3.25)

N.D.

N.D.

Striatum

0.03 ± 0.01

0.07 ± 0.03 (2.33)

0.06 ± 0.01 (2.00)

N.D.

N.D.

Lbp

MAVd

2.22 ± 0.86

3.52 ± 2.43 (1.58)

1.40 ± 0.48 (0.63)

12.05 ± 7.42 (5.06)

2.38 ± 1.12

Reg3a

MAVa,d

0.001 ± 0.001

0.45 ± 0.50 (450.00)

0.01 ± 0.01 (10.00)

N.D.

N.D.

Parietal Cortex

0.001 ± 0.001

0.002 ± 0.001 (2.00)

0.005 ± 0.00 (5.00)

N.D.

N.D.

Striatum

0.01 ± 0.01

0.02 ± 0.01 (2.00)

0.03 ± 0.004 (3.00)

N.D.

N.D.

Reg3b

MAVa,d

0.001 ± 0.001

0.21 ± 0.27 (210.00)

0.01 ± 0.004 (10.00)

0.44 ± 0.36 (62.86)

0.007 ± 0.014

Parietal Cortex

0.001 ± 0.00

0.001 ± 0.00 (1.00)

0.001 ± 0.00 (1.00)

N.D.

N.D.

Striatum

0.0005 ± 0.0001

0.0007 ± 0.0002 (1.40)

0.0005 ± 0.0003 (1.00)

N.D.

N.D.

Reg3g

MAVa

0.003 ± 0.002

0.31 ± 0.33 (103.33)

0.02 ± 0.01 (6.67)

0.19 ± 0.26 (47.50)

0.004 ± 0.005

Sct

MAVa,d

0.004 ± 0.001

0.025 ± 0.017 (6.25)

0.006 ± 0.002 (1.50)

0.014 ± 0.004 (3.50)

0.004 ± 0.002

Slc15a1

MAVa,c

0.19 ± 0.09

3.19 ± 5.72 (16.79)

0.16 ± 0.07 (0.84)

0.04 ± 0.04 (1.33)

0.03 ± 0.02

Parietal Cortex

0.03 ± 0.003

0.04 ± 0.01 (1.33)

0.03 ± 0.003 (1.00)

N.D.

N.D.

Striatum

0.04 ± 0.01

0.05 ± 0.01 (1.25)

0.04 ± 0.01 (1.00)

N.D.

N.D.

Sox18

MAVa

5.57 ± 2.58

0.96 ± 0.33 (0.17)

2.11 ± 1.54 (0.38)

N.D.

N.D.

Parietal Cortex

0.36 ± 0.06

0.17 ± 0.07 (0.47)

0.24 ± 0.20 (0.67)

N.D.

N.D.

 

Striatum

0.46 ± 0.10

0.23 ± 0.08 (0.50)

0.26 ± 0.25 (0.56)

N.D.

N.D.

  1. All genes are expressed as a percentage of Gapdh and are shown as the mean plus/minus standard error of the mean. Fold-changes of treatment to control are given in parentheses at the 3 hr time point. At the 1 day time point, fold-changes (in parentheses) are expressed as AMPH to EIH. All the RT-PCR primers appeared to function optimally except those for Slc15a1 which gave a somewhat bimodal melt curve (several sets of primers were tried). The number of RNA aliquots (one per animal) used to determine expression per treatment group were as follows: 3 hr control, n = 6; 3 hr AMPH, n = 7; 3 hr EIH, n = 6; 1 day AMPH, n = 6; 1 day EIH, n = 6.
  2. N.D. designates expression not determined.
  3. Symbols indicating statistically significant difference between treatments are found as a superscript after the brain region (second column).
  4. a AMPH significantly differs from control at 3 hr (p < 0.05).
  5. b EIH significantly differs from control at 3 hr (p < 0.05).
  6. c AMPH significantly differs from EIH at 3 hr (p < 0.05).
  7. d AMPH significantly differs from EIH at 1 day (p < 0.05).