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Table 7 RT-PCR determination of gene expression in the choroid plexus

From: Comparison of the global gene expression of choroid plexus and meninges and associated vasculature under control conditions and after pronounced hyperthermia or amphetamine toxicity

 

3 hr

1 day

NCBI gene symbol

Control

AMPH

EIH

AMPH

EIH

Apold1(Verge)a

0.03 ± 0.002

0.06 ± 0.02 (2.00)

0.06 ± 0.07 (2.00)

N.D.

N.D.

Bmp4 a,c

0.55 ± 0.04

3.65 ± 1.26 (6.64)

0.84 ± 0.23 (1.53)

N.D.

N.D.

Cebpd a,b

0.01 ± 0.003

0.24 ± 0.09 (24.00)

0.15 ± 0.13 (15.00)

N.D.

N.D.

Cldn1

5.74 ± 0.70

5.73 ± 0.98 (1.00)

6.49 ± 0.80 (1.13)

N.D.

N.D.

Cxcl1 a,b

0.004 ± 0.002

0.40 ± 0.62 (100.00)

0.30 ± 0.51 (75.00)

N.D.

N.D.

Cyr61 a,b

0.24 ± 0.06

3.54 ± 2.70 (14.75)

3.16 ± 5.10 (13.17)

N.D.

N.D.

Dio2 a

0.01 ± 0.005

4.34 ± 2.03 (434.00)

1.08 ± 1.13 (108.00)

N.D.

N.D.

Edn1 b

0.003 ± 0.001

0.005 ± 0.002 (1.67)

0.011 ± 0.010 (3.67)

0.003 ± 0.004 (1.50)

0.002 ± 0.000

Fst b,d

0.03 ± 0.02

0.06 ± 0.02 (2.00)

0.10 ± 0.06 (3.33)

0.03 ± 0.02 (3.00)

0.01 ± 0.003

Has1 a,b

0.09 ± 0.06

0.17 ± 0.03 (1.89)

0.17 ± 0.02 (1.89)

0.11 ± 0.02 (1.10)

0.10 ± 0.02

Lbp a

0.11 ± 0.06

0.53 ± 0.32 (4.81)

0.24 ± 0.12 (2.18)

0.97 ± 0.57 (2.11)

0.46 ± 0.31

Nat3 b

0.01 ± 0.004

0.01 ± 0.004 (1.00)

0.01 ± 0.008 (1.00)

0.004 ± 0.003 (0.50)

0.008 ± 0.010

Nos3

0.06 ± 0.03

0.09 ± 0.04 (1.50)

0.10 ± 0.06 (1.67)

0.08 ± 0.05 (1.33)

0.06 ± 0.01

Ocln a

4.96 ± 0.52

7.24 ± 1.12 (1.46)

6.01 ± 1.76 (1.21)

N.D.

N.D.

Reg3b

N.E.

N.E.

N.E.

N.E.

N.E.

Sct

N.E.

N.E.

N.E.

N.E.

N.E.

Sectm1b a

0.01 ± 0.002

0.46 ± 0.15 (46.00)

0.15 ± 0.14 (15.00)

N.D.

N.D.

  1. All genes are expressed as a percentage of Gapdh, and are shown as the mean plus/minus standard error of the mean. Fold-changes of treatment to control are given in parentheses at the 3 hr time point. At the 1 day time point, fold-changes (in parentheses) are expressed as AMPH to EIH. The number of RNA aliquots (two to three animals per aliquot) used to determine expression per treatment group were as follows: 3 hr control, n = 7; 3 hr AMPH, n = 7; 3 hr EIH, n = 7; 1 day AMPH, n = 8; 1 day EIH, n = 7.
  2. N.E. means gene not expressed (mean Ct value > 35).
  3. N.D. designates expression not determined.
  4. Symbols indicating statistically significant difference between treatments are found as a superscript after the gene name (first column).
  5. a AMPH significantly differs from control at 3 hr (p < 0.05).
  6. b EIH significantly differs from control at 3 hr (p < 0.05).
  7. c AMPH significantly differs from EIH at 3 hr (p < 0.05).
  8. d AMPH significantly differs from EIH at 1 day (p < 0.05).