Identification of transcription start sites (TSS) in the S. meliloti genome. (a) Schematic showing parameters of TSS types based on the minimal transcription unit model described in Methods. (i) TSS of an mRNA. (ii) TSS of a leaderless transcript. (iii) TSS of a putative mRNA. (iv) TSS of a sense transcript. (v) TSS of a cis-encoded antisense RNA. (vi) TSS of a trans-encoded sRNA. (b) Strand and replicon specific distribution of TSS by category. The y-axis indicates log10 coverage for mapped reads. (c) Number of protein-coding genes, asRNAs and sRNAs possessing 1, 2, 3, 4 or >4 TSS. The last two rows give the number of genes or IGRs possessing 1, 2, 3, 4 or >4 asRNAs or sRNAs. (d) TSS associated with pstS, ctrA, and SMc02853 (indicated by blue triangles). Putative transcription factor binding motifs are shown as orange rectangles. (e) TSS associated with SMb_asRNA_3039 and SMa_sRNA_136 (indicated by blue triangles).