Alignments of the N-terminal domains of GNBP/GRPs. The deduced amino acid sequences of N. lugens GRPs were compared with D. melanogaster GNBP1 (CAJ18915), GNBP2 (CAJ19023), GNBP3 (AF228474), B. mori GRP1 (BAA92243) and GRP3 (BAG70413). The putative signal peptides are underlined. The amino acids in orange and green shade indicate the conserved and type-conserved residues, respectively. The predicted secondary structural elements of eight β-strands are shown below the alignments. DmGNBP, D. melanogaster gram-negative bacteria binding proteins; BmGRP, B. mori β-1, 3-glucan recognition protein.