Figure 4

Increased miR-155 and Kras expression suggest a possible cancer predisposition in G1 phase WT cells. (A) Transcriptional regulation of known oncogenes and tumor suppressors by microarray at 2 hours post IR in WT cells (fold change) (also shown are values of the fold difference after RAG-induced damage). (B) Gene expression of miR-155 and Socs1 mRNA by qRT-PCR at 2, 4 and 8 hours post IR. Each data point is the final ddC_T calculated fold change value of 1 Gy/0 Gy from 3 WT pre-B cells lines, with 3 to 5 technical replicates each, following normalization to U6 or 18S, respectively.