Figure 4

Inactivation of 10 lipid genes by RNAi dramatically changed fat storage in C . elegans . Late L4 worms were fixed with paraformaldehyde and stained with Nile Red [48]. Images were captured using identical settings and exposure time for each image. Inactivation of sams-1, mboa-7, and C05D11.7 led to increased lipid size; RNAi knockdown of dsh-16 and let-767 slightly though noticeably increased the size of lipid droplets in C. elegans; Meanwhile, RNAi inactivation of lpin-1, sptl-1, acs-1, cyp-29A2 and fat-6 resulted in significantly decreased fat storage and comparatively smaller size of lipid droplets. Bar, 10 μm. Arrow indicates the lipid droplet.