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Table 2 A set of 11 multiplex panels including the 30 most informative ruzigrass microsatellite markers

From: Development and validation of microsatellite markers for Brachiaria ruziziensisobtained by partial genome assembly of Illumina single-end reads

Panel

Marker

Dye

Forward Primer

Reverse Primer

Allele No

Observed size ranges

He

Ho

PIC

1

Brz0182

NED

ACGTTATTGGACTTGGGTGA

AGCCTGACCAAATTCTTGTG

10

252-328

0.823

0.545

0.868

 

Brz0097

HEX

TAATTTGTTCCACCCACAGG

GTGACAGAGTTCGGGAGCTA

5

234-242

0.705

0.375

0.747

2

Brz0075

6-FAM

GAAGCTGCAAAGGCTGAGT

GGAGGAGAGAGAAGAGCAAGA

8

129-153

0.809

0.727

0.839

 

Brz0148

6-FAM

GCTCTTGACCTTGACGATGT

TGCACTTGAGAGAGACGAAA

8

248-274

0.787

0.909

0.800

 

Brz0083

HEX

CATGATATTTGCCTGTCAAGG

AGCACCGGTGATGTGAATA

6

233-249

0.765

0.778

0.788

3

Brz0017

HEX

TTCCATTTATTTGCCTGTTCA

ATTTTCCCTATCCGACCTTTC

11

134-160

0.840

1000

0.864

 

Brz0116

HEX

TCAAGAAATGGACTCCCAAA

TCTAGGTCATGCAAGCCATT

9

223-271

0.803

0.900

0.827

 

Brz0047

6-FAM

TGTGAGACATAAACCATTGGAA

AATGGGTGCTGGAAATGTAAC

7

150-170

0.731

0.556

0.762

4

Brz0021

HEX

CAGCTGAAAGTTCCCAAAAAT

CTGAATGATAAAGGGTGCAAA

9

151-183

0.770

0.400

0.816

 

Brz0087

NED

TTCCCCCACTACTCATCTCA

AACAGCACACCGTAGCAAGT

6

239-273

0.716

1000

0.748

5

Brz0065

6-FAM

AGCTAAGCAAATTTCAAGAACG

TAATGTGGAACATTGCCCTAA

12

130-166

0.829

0.700

0.875

 

Brz0130

6-FAM

TCCTTTCATGAACCCCTGTA

CATCGCACGCTTATATGACA

9

242-266

0.820

0.636

0.858

 

Brz0131

HEX

TGCAATGACATTAAATCAACC

GCTGCAACACAAACAAAATAA

6

254-264

0.712

0.714

0.744

6

Brz0147

HEX

CTGAGGACGCTCCTACTGAA

TTGATTTCAACACCCCAACT

10

240-288

0.825

0.700

0.868

 

Brz0031

6-FAM

CCCCCATTTAACACCATAGTT

GCTCAAAATGCAATGTACGTG

7

144-156

0.770

0.667

0.804

7

Brz0177

6-FAM

TGGAGTTGAGGCTTTAGGAA

GTGTTTGGAAACCACTTGCT

6

291-319

0.725

0.125

0.795

 

Brz0107

6-FAM

AGAGGAATTGACTTGGAAAAA

GCATGCACGTAAATTTTCACT

6

227-247

0.747

0.444

0.788

 

Brz0004

6-FAM

TTGTTGTGGTACACCGGTACT

CAAAACCTGAATCACCATGTC

6

113-155

0.703

0.222

0.745

8

Brz0118

NED

AGGAGGTCCAAATCACCAAT

CGTCAGCAATTCGTACCAC

10

237-263

0.812

0.636

0.849

 

Brz0219

HEX

GCAGTTCTTGCTTTTTCAGG

TCTCCTTATGCAAGGCTTC

6

294-304

0.768

0.818

0.778

 

Brz0156

6-FAM

GCCATGATGTTTCATTGGTT

TTTTGCACCTTTCATTGCTT

7

239-265

0.752

0.636

0.770

9

Brz0142

6-FAM

GCTGGGTTATGCTAATGCAA

TCAAGCATGAACATTGAAACA

10

241-287

0.823

0.875

0.871

 

Brz0180

HEX

CACACGGTCCATCTTGATTT

TCCATAATGCATTGTCTTGAAA

7

285-305

0.751

0.091

0.800

 

Brz0089

NED

CAAACCTATTCCACGGTCAA

TGGACAATGCTATTCAAACG

7

224-248

0.710

0.571

0.759

10

Brz0048

HEX

GAATCTAAGCAGCGGATCAAT

TCACAAGAAGGTCCTCACAAG

9

139-161

0.813

0.818

0.839

 

Brz0206

NED

GAAGTGGCAAGACACACACA

TGAGCTTTTCGTCTCTCCTG

7

278-302

0.757

0.600

0.783

 

Brz0038

6-FAM

CTGAAAATAAGAGCCGTCCAT

ATAAGGTGAGCCACAACTGAG

6

140-154

0.772

0.909

0.778

11

Brz0171

6-FAM

TTGTCTCACTTGTGCACTCC

GCTAGCAGGTAGCAAGATGG

7

312-348

0.725

0.250

0.787

 

Brz0015

6-FAM

AATAGAAAACGTGAGCCCATT

TCCACCAATATGATTCAAACG

6

144-156

0.764

0.636

0.783

 

Brz0152

NED

ATGCTGCACTTACTGGTTCA

GGCTATCAATTCGAAGACCA

6

228-248

0.748

0.667

0.774