Deep sequencing for de novo construction of a marine fish (Sparus aurata)transcriptome database with a large coverage of protein-coding transcripts

Table 1 Statistics for 454 pyrosequencing libraries

	Skeletal muscle	Intestine	Blood	HK-38	HK-105
Pyrosequencing reads
High-quality reads	447,166	614,899	576,796	577,112	729,941
Average read length (bp)	294	304	290	349	320
Total Megabases	131.5	186.9	167.3	201.4	233.6
Assembly statistics
Number of contigs	7,808	9,475	12,003	14,008	12,474
Reads assembled	314,628	482,080	389,934	392,195	508,826
Average contig length (bp)	968	1,090	831	1,059	1,148
Number of singletons	48,750	80,527	114,984	105,431	91,885
Average singleton length (nt)	316	298	284	318	334
Total consensus Megabases	23.0	34.3	42.7	48.4	45.0
Average sequences coverage	6.4	6.3	4.0	4.2	5.8

Sequence characteristics of the five 454 normalized libraries derived from skeletal muscle, intestine, blood and head kidney (HK-38, early parasite infection; HK 105, chronic parasite infection).

ISSN: 1471-2164