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Table 10 Filtration process results for the 47,921 sequences with oligos in forward and reverse orientation

From: A combined strategy involving Sanger and 454 pyrosequencing increases genomic resources to aid in the management of reproduction, disease control and genetic selection in the turbot (Scophthalmus maximus)

 

Forward

1stfiltration

Both systems

Only immune

Only reproduction

Without signal

Total

Reverse

Both systems

12,189

275

4,297

5,276

22,037

Only immune

531

74

71

116

792

Only Reproduction

6,131

47

2,536

1,946

10,660

Without signal

8,310

179

3,119

2,194

13,802

  Total

27,161

575

10,023

9,532

47,291

 

2 nd filtration

     

Reverse

Both systems

2,976

153

1627

5,493

10,249

 

Only immune

298

85

86

257

726

 

Only reproduction

2,332

61

1,360

3,112

6,865

 

Without signal

9,059

359

4,812

15,221

29,451

 

  Total

14,665

658

7,885

24,083

47,291

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BMC Genomics

ISSN: 1471-2164

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