Figure 6

Increased expression of genes involved in macrophage activation and antigen presentation occurs in RSV-infected macrophage cells. (A) The fold change (FC) in the expression of cytokine at 4 and 24 hours post-infection (hpi) are compared with that in mock-infected cells as a heat map. In each case, the FC range, probe identification (Probe ID), and gene name (Gene Title) are indicated. (B) RSV-infected PMΦ cells were stained with anti-RSV and examined by fluorescence microscopy at (i) 24 hpi, 48 hpi and 72 hpi and (ii) 96 hpi. The stained cells were examined using either the same camera exposure time or at a reduced exposure time (highlighted by *) to enable the inclusion bodies (IB) to be viewed (at magnification x20). Inclusion bodies are highlighted (white arrow) and a more diffuse anti-RSV staining pattern is highlighted (*). (iii) RSV-infected HEp2 cells stained using anti-RSV at 24 hpi. The IBs are highlighted (white arrow). (C) At 48 hpi RSV-infected PMΦ cells were stained with anti-RSV and anti-LAMP-1. The inclusion bodies (IB) and the LAMP-1 punctate staining pattern (*) are highlighted (D). Also shown is the co-staining pattern of anti-RSV and anti-LAMP-1 in the diffusely stained phagosomes (*) that predominates latter in infection.