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Figure 4 | BMC Genomics

Figure 4

From: Intron retention and transcript chimerism conserved across mammals: Ly6g5b and Csnk2b-Ly6g5b as examples

Figure 4

Human CSNK2B, LY6G5B and CSNK2B-LY6G5B chimeric protein characterisation. Schematic representation of A) V5 and Hisx6 double tag strategy. V5 (red box) and Hisx6 (yellow box) epitopes are shown. Exon numbers are indicated. B) Western-blot analysis showing in vitro expression of CSNK2B, LY6G5B and CSNK2B-LY6G5B chimera double tagged proteins. GAPDH expression is shown as internal control. C) Immunofluorescence experiments in permeable (P) and non permeable (NP) conditions showing CSNK2B, LY6G5B and CSNK2B-LY6G5B chimeric protein cellular distribution.

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BMC Genomics

ISSN: 1471-2164

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