Table 2 Expression constructs primers

Long primers for V5 epitope cloning in pcDNA3.1/Zeo(-)

Name

Sequence

MCV5_F01

GGGGCGGCCGCATGGGAAAGCCGATCCCAAACCCTCTATTAGGTCTGGACTCCACCGGATCCTGAGATATCGGG

MCV5_R01

CCCGATATCTCAGGATCCGGTGGAGTCCAGACCTAATAGAGGGTTTGGGATCGGCTTTCCCATGCGGCCGCCCC

Primers for CSNK2B, LY6G5B and CSNK2B-LY6G5B Chimera ORFs cloning in pcDNA3.1/V5-His-TOPO

Name

Sequence

HsCSNK2BORF_F01

CCCGGATCCATGAGCAGCTCAGAGG

HsCSNK2BORF_R01

CCCACCGGTGCGAATCGTCTTGAC

HsLY6G5BORF_F01

CCCGGATCCATGAAGGTCCATATGC

HsLY6G5BORF_R01

CCCACCGGTGGAAGGGTGAGGTGTC

Primers for final construct in pcDNA3.1/Zeo(-)

Name

Sequence

HsCSNK2BORF_F01

CCCGGATCCATGAGCAGCTCAGAGG

HsLY6G5BORFE2_F02

CCCGGATCCGTTCCTGTTCCCGACATC

Hisx6HindIIIR_01

CCCAAGCTTTCAATGGTGATGGTGATGATG

HsLY6G5BPS_F01

GGGTCTAGAATGAAGGTCCATATGC

HsLY6G5BPS_R01

CCCGCGGCCGCTCTTTCCTACTGTGAA