Strains
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Streptococcus infantarius subsp. infantarius
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CJ18
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Wild type strain, suusac isolate
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[2, 7, 31]
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CJ18/pVE6007
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CJ18 derivative carrying pVE6007, CmR
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this study
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CJ18ΔlacIIC
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lacIIC::pLFB1005, lacIIC gene disruption derivative of CJ18, EmR
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this study
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CJ18ΔlacZ
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lacZ::pLFB1006, lacZ gene disruption derivative of CJ18, EmR
|
this study
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CJ18ΔlacS
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lacS::pLFB1007, lacS gene disruption derivative of CJ18, EmR
|
this study
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Lactococcus lactis
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LL302
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RepA+ derivative of MG1363, host for pORI28
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[51]
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Plasmids
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pORI28
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EmR, Ori+, RepA-, pWV01 derivative, vector for chromosomal insertions in Gram-positive bacteria
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[52]
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pVE6007
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CmR, thermosensitive derivative of pWV01, carrier plasmid for pORI28
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[53]
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pLFB1005
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EmR, pORI28 derivative containing a 939-bp internal fragment of lacIIC.
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this study
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pLFB1006
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EmR, pORI28 derivative containing an 1177-bp internal fragment of lacZ.
|
this study
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pLFB1007
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EmR, pORI28 derivative containing a 900-bp internal fragment of lacS.
|
this study
|