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Table 3 Strains and plasmids used in this study

From: Comparative genome analysis of Streptococcus infantarius subsp. infantariusCJ18, an African fermented camel milk isolate with adaptations to dairy environment

Material Relevant featuresa Source
Strains  
Streptococcus infantarius subsp. infantarius
CJ18 Wild type strain, suusac isolate [2, 7, 31]
CJ18/pVE6007 CJ18 derivative carrying pVE6007, CmR this study
CJ18ΔlacIIC lacIIC::pLFB1005, lacIIC gene disruption derivative of CJ18, EmR this study
CJ18ΔlacZ lacZ::pLFB1006, lacZ gene disruption derivative of CJ18, EmR this study
CJ18ΔlacS lacS::pLFB1007, lacS gene disruption derivative of CJ18, EmR this study
Lactococcus lactis  
LL302 RepA+ derivative of MG1363, host for pORI28 [51]
Plasmids   
pORI28 EmR, Ori+, RepA-, pWV01 derivative, vector for chromosomal insertions in Gram-positive bacteria [52]
pVE6007 CmR, thermosensitive derivative of pWV01, carrier plasmid for pORI28 [53]
pLFB1005 EmR, pORI28 derivative containing a 939-bp internal fragment of lacIIC. this study
pLFB1006 EmR, pORI28 derivative containing an 1177-bp internal fragment of lacZ. this study
pLFB1007 EmR, pORI28 derivative containing a 900-bp internal fragment of lacS. this study
  1. aCmR Chloramphenicol resistant; EmR Erythromycin resistant.