Comparative genome analysis of Streptococcus infantarius subsp. infantariusCJ18, an African fermented camel milk isolate with adaptations to dairy environment

Table 3 Strains and plasmids used in this study

Material	Relevant features^a	Source
Strains
Streptococcus infantarius subsp. infantarius
CJ18	Wild type strain, suusac isolate	[2, 7, 31]
CJ18/pVE6007	CJ18 derivative carrying pVE6007, Cm^R	this study
CJ18ΔlacIIC	lacIIC::pLFB1005, lacIIC gene disruption derivative of CJ18, Em^R	this study
CJ18ΔlacZ	lacZ::pLFB1006, lacZ gene disruption derivative of CJ18, Em^R	this study
CJ18ΔlacS	lacS::pLFB1007, lacS gene disruption derivative of CJ18, Em^R	this study
Lactococcus lactis
LL302	RepA⁺ derivative of MG1363, host for pORI28	[51]
Plasmids
pORI28	Em^R, Ori⁺, RepA^-, pWV01 derivative, vector for chromosomal insertions in Gram-positive bacteria	[52]
pVE6007	Cm^R, thermosensitive derivative of pWV01, carrier plasmid for pORI28	[53]
pLFB1005	Em^R, pORI28 derivative containing a 939-bp internal fragment of lacIIC.	this study
pLFB1006	Em^R, pORI28 derivative containing an 1177-bp internal fragment of lacZ.	this study
pLFB1007	Em^R, pORI28 derivative containing a 900-bp internal fragment of lacS.	this study

ISSN: 1471-2164