Skip to main content

Table 4 Oligonucleotides used to amplify internal fragments of target genes to construct knock-out strains

From: Comparative genome analysis of Streptococcus infantarius subsp. infantariusCJ18, an African fermented camel milk isolate with adaptations to dairy environment

Name

Sequence (5’ to 3’)a

Name

Sequence (5’ to 3’)

lacS_for

GATCGGATCCGATCCAAAGCAAAATAGTCA

lacS_con_for

TCCTATGCAGCGGGTGCTT

lacS_rev

GATCGAATTCTGCAGTCAAGATAATTGGA

lacS_con_rev

GAGATAATCATAAGGATAACAA

lacZ_for

GATCCTGCAGGCGTTAATACAGTTGACGCTCAC

lacZ_con_for

TTACTTAAACGATCCAAAGA

lacZ_rev

GATCGGATCCTTTGCCATGTACCGTGTGTT

lacZ_con_rev

CATGTTATTGGCACGATCCA

lacIIC_for

GATCGGATCCAATATTTGCGAGCGATTCGT

lacIIC_con_for

GGAAACCATTCTTTGAGAG

lacIIC_rev

GATCGAATTCTACAATTGGAGCACCGAACA

lacIIC_con_rev

ATTTGAAGATCCACACGTT

pORI_for

TTG ATA ATG AAC TGT GCT GA

pORI _rev

ACG AAT CGC CAA CGT TTT CG

  1. a) Endonuclease restriction sites introduced in primers are underlined.