Table 1 Characterization of mitochondrial enrichment and intactness
From: The genome and transcriptome of perennial ryegrass mitochondria
Parameters | *Fraction | ||
|---|---|---|---|
Homogenate | Crude mitochondria | ||
Protein | Total (mg) | 731 ± 40 | 42.0 ± 7 |
CCO activity | Total (μmol min-1) | 13.0 ± 2.90 | 5.87 ± 1.41 (45%) |
Specific (nmol min-1 mg-1) | 18.0 ± 2.83 | 139 ± 9.90 (7.7-fold) | |
Latency (%) | 90.0 ± 1.41 | 89.5 ± 0.71 | |
MDH activity | Total (μmol min-1) | 214 ± 61.5 | 26.5 ± 0.71 (12%) |
Specific (nmol min-1 mg-1) | 291 ± 68.6 | 639 ± 91.2 (2.2-fold) | |
Latency (%) | 22.0 ± 1.41 | 77.5 ± 0.71(3.5-fold) | |
Yield of mtDNA | Total (μg) | - | 3.47 ± 0.27 |