Figure 2

Visualization of RNA-Seq data in the CLC genomic workbench software. A. Reads from the RNA-Seq data of wt PH-1 and mutant ebr1 were mapped to the gene FGSG_04412. 168 reads in PH-1 and 216 reads in ebr1 uniquely matched to the exon of FGSG_04412. In contrast, no reads matched to the intronic regions. Y-axis represents the coverage number of reads of each nucleotide. B. Analysis of the reads matching to the EBR1 gene in wt PH-1 and mutant ebr1, respectively. 75 reads from wt PH-1 RNA-Seq data matched to EBR1, whereas no reads from mutant ebr1 RNA-Seq data matched to EBR1.