Figure 6

Alternative splicing is developmentally regulated. RNA samples were isolated from wt PH-1 isolate at five different time points (0 h, 2 h, 8 h, 24 h, and 36 h after incubation of conidia in liquid CM medium-containing shake cultures). RT-PCRs were performed at the different time points for four alternatively spliced genes (FGSG_00303, FGSG_06760, FGSG_05122 and FGSG_04141). Genomic DNA template was used as control. Capital letter R represents reference transcript isoform. Capital letter A represents alternatively spliced transcript isoform. The experiment was independently performed twice with similar results.