Figure 3

Differential gene expression analysis in C. albicans and C. dubliniensis. (A) Differential expression of orthologs (= DEO) between C. albicans and C. dubliniensis in YPD supplemented with 10% FCS. Blue triangles represent orthologous pairs with a significantly higher expression in C. albicans than in C. dubliniensis while those marked with yellow circles show higher expression in C. dubliniensis. (C) Yeast to hyphae (Y-t-H) switch in C. albicans. Blue triangles represent hyphally up regulated genes in YPD supplemented with 10% FCS while down regulated genes are marked with blue circles. (E) Yeast to hyphae (Y-t-H) switch in C. dubliniensis. Yellow triangles represent hyphally up regulated genes in water supplemented with 10% FCS while down regulated genes are marked with yellow circles. All marked genes are significantly differentially regulated with an adjusted p-value < 0,001 and a |FC| > 4. FC = fold change. (B, D, F) GO enrichment analysis of differentially expressed genes from the DEO analysis (B), the yeast to hyphae (Y-t-H) transition analysis in C. albicans (D) and in C. dubliniensis (F). Corresponding sets of up and down regulated genes were mapped with the “GO term finder” at CGD for two ontologies-biological process and cellular component. The x-fold enrichment is calculated as the ratio of percentages of the cluster frequency of the tested gene set and the cluster frequency of the genomic background. Representative GO terms were taken to illustrate trends of enrichment since most of the genes are assigned to more than one term.