Figure 2

Pattern matching algorithm reveals genes with pulsatile expression. A pattern matching algorithm revealed pulsatile expression patterns of 11 probes in LD heads and 5 probes in LD bodies that were rhythmic with a c > 1.6 and peak-to-trough fold change greater than 1.5 (c is the convolution value between probe signals and the pulsatile template). Two of these genes from LD bodies and five from LD heads had not been previously identified as rhythmic under those conditions [30]. (A) Hierarchical clustering of genes found rhythmic using the pattern matching algorithm in LD heads (top) and bodies (bottom). Red indicates higher expression, and green indicates lower expression versus the mean value for each gene. (B) Gene expression profile from microarray data of one of the new genes found rhythmic in LD heads, cyptochrome P450 6M2 (CYP6M2). (C) Quantitative real-time RT-PCR (qRT-PCR) validates microarray analysis gene-expression profile of the pulsatile expression of CYP6M2 in LD heads. Data are mean ± SD compared with expression of RPS7. Day and night are indicated by the horizontal white/black bars.