Figure 1

Transcriptome analysis of UVB-induced senescence. A. Ingenuity pathway analysis. Activated pathways were determined by Ingenuity System Pathway Analysis software (http://www.ingenuity.com/) by Core Analysis. Shown are eight significantly activated pathways (threshold p-value ≤ 0.05). B. In silico analysis of mRNA expression change in response to UVB irradiation. (left) The differential gene expression profile from total RNA was examined by Affymetrix GeneChip analysis (cutoff of > 1.5 fold regulation) 1, 7, and 9 days between control and UVB irradiated of HDF samples. The expression values were sorted by level of Ezh2 expression (rows). (right) Heatmap of selected 67 genes were determined by RT-PCR and sorted by level of Ezh2 expression (rows), red representing overexpression and green representing underexpression of the transcript.