Figure 6

Correlation network of miR-101 and its high confidence target genes. (A) We used 10 prediction tools to obtain, based on public data, candidate miRNA-mRNA target interactions and we identified high confidence targets by mRNA and miRNA expression. We calculated Pearson correlation coefficients between miRNAs and their targets. Results of the analysis are presented here for miR-101. The color and shape of nodes are based on different node attributes available for the analyzed dataset. The red triangles, purple circles and orange diamonds in the network are indicating miRNAs, target genes, and transcription factors, respectively. Edges represent correlation between miRNAs and mRNAs, the color of the edges designate the type of interaction. Red is for positive and green is for negative correlation. (B) Protein samples for the indicated time points were collected as described (left panel). Protein levels were determined for Ezh2 by standard Western blot analysis. Experiments were performed in triplicates, shown here is a representative result. miR-101 levels were determined by real-time qPCR as described (right panel). Data represents the mean ± SE for three independent experiments. Co: untreated controls. *p < 0.01; **p < 0.001.