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Figure 2 | BMC Genomics

Figure 2

From: High-throughput genome sequencing of lichenizing fungi to assess gene loss in the ammonium transporter/ammonia permease gene family

Figure 2

Heterologous expression of Cladonia grayi AMTP genes in a strain of Saccharomyces cerevisiae lacking AMTPs. Left: Ten-fold serial dilutions (107 to 103 cells/ml) of a S. cerevisiae (Sc) AMTP triple knockout complemented with AMTPs from the lichenizing fungus Cladonia grayi (Cg). Left, limiting ammonium (5 mM); right, control plate with excess ammonium (20 mM). Top to bottom, 1–6, transformants of MLY131 (a/α) carrying a centromeric plasmid (p416-GPD) containing the following genes expressed the constitutive GPD promoter: 1. CgMep1a, 2. CgMep1b, 3. CgMep3, 4. CgMep2, 5. Plasmid only (negative control) 6. Saccharomyces cerevisiae Mep2 (positive control).

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BMC Genomics

ISSN: 1471-2164

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