Expression of 4 synthase genes in response to mechanical wounding and methyl jasmonate (MJ) treatment. (A) In the stem of 3-year-old A. sinensis trees, mechanical wounding increased the mRNA levels of 4 genes that encode enzymes in the sesquiterpene biosynthesis pathway. Each value is the mean ± SE of 3 independent biological determinations. CK: healthy 3-year-old control trees; 5d: 3-year-old trees pruned after 5 days. (B) In A. sinensis calli, treatment with 100 μM MJ increased the mRNA levels of 4 genes that encode enzymes in the sesquiterpene biosynthesis pathway. A. sinensis calli were transferred to MJ-containing medium and cultivated for 5 days before being sampled for analysis. Each value is the mean ± SE of 3 independent biological determinations, and the data shown are relative ratios. The genes were named according to their functional annotation. CK: healthy calli controls; MJ: calli treated with MJ for 5 days.