Figure 2

RNAseq (SAGE) based comparative analysis of gene expression in liver tissue of H. sapiens, M. fascicularis, S. scrofa, M. auratus and R. norwegicus. 48 genes from the public domain network REACTOME “Lipid digestion, mobilization, and transport” were selected to highlight species specific differences in gene expression relevant for HDL biosynthesis (left panel). Gene clusters generated by hierarchical clustering are labelled as CL1 to CL6. The highly abundant albumin transcript is marked. The right panel shows mRNA levels of a standard reference set of liver housekeeping genes compiled from public domain data (L. Badi, unpublished). The log2 values of normalized read counts are presented in a standard heat map as indicated. Grey fields indicate genes lacking valid expression data. SAGE tags of cluster 6 transcripts were quantified using the Chinese hamster genome draft as resource because they had no matches in the 454 library due to low abundance. The RNA source for each species is given in the material section of the paper. All SAGE libraries were built using commercial kits and the source of tissue is given under Methods. Abbreviations of organisms included are indicated at the bottom.