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Figure 7 | BMC Genomics

Figure 7

From: Genome-wide identification, classification and expression profiling of nicotianamine synthase (NAS) gene family in maize

Figure 7

Histochemical localization of ZmNAS1;1/1;2 in maize seedlings under Fe sufficient and deficient status. In situ hybridization was performed on shoot and root sections of maize seedlings under Fe sufficient (upper panel) and deficient (lower panel) conditions with digoxigenin-labeled antisense or sense probes. Longitudinal sections of shoot apex (A, B, E and G), and transverse sections of root (C, D, F and H) were hybridized. The expression of ZmNAS1;1/1;2 was observed as purple staining in cortex and stele of Fe sufficient roots (C), epidermis, cortex and stele of Fe deficient roots (F), and leaf primordia of shoot apices (E) detected with antisense probes. No signal was observed in either control sections with sense probes (B, D, G and H) or shoot apices of Fe sufficient seedlings detected with antisense probes (A). Arrow indicates leaf primordia (E) and epidermis of roots (C and F). Pi, pith; Mx, Metaxylem; Co, cortex; Ep, epidermis; Lp, leaf primordia. The length of bars corresponds to 100 μm.

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