Figure 8

Histochemical localization of ZmNAS3 in maize shoots. In situ hybridization was performed on longitudinal sections of shoot (A, B and C) and transverse sections of leaf (E, D and F) using digoxigenin-labeled antisense or sense probes. The hybridization signal was localized in the leaf primordia (A), axillary meristem (B) and mesophyll cells (D and E), while no signal was detectable in control sections hybridized with sense probes (C and F). Arrow indicates the hybridization signals represented by purple staining. Lp, leaf primordia; Am, axillary meristem; Mc, mesophyll cells; Vb, vascular bundles. The length of bars corresponds to 100 μm.