Figure 1

Examples illustrating different modes of alternative transcript generation for C. elegans transcription factor genes. A. Alternative promoters with unique starting exons for crh-2. B. Alternative promoters with nested transcripts for fkh-9. C. Alternative transcript ends for nhr-104. The non-coding RNA genes R11E3.14 and R11E3.16 are transcribed in the opposite direction. D. Inclusion or exclusion of an intron for pqn-21. E. Inclusion or exclusion of an exon for nhr-117. For each example, the molecular gene names are included in brackets after the genetic gene name, with the additional final letter (a/b/c) distinguishing the transcripts encoding distinct isoforms. The triangles indicate positions of gfp insertion used to tag expression for different transcripts. The grey triangle indicates that gfp was inserted with an extra nucleotide upstream to disrupt the translational reading frame, and therefore reporter expression, for other transcripts starting further upstream. The crosses indicate where translational reading frames of protein-coding regions were disrupted by the insertion of single base pairs to eliminate reporter expression arising from particular transcripts for fusion genes with gfp inserted at the end of the protein coding region. The scale bar in each panel is in base pairs along the respective chromosome.