Figure 3

Example fluorescence micrographs for expression of fkh-9::gfp fusions. A. An L3 larva of strain UL3218 with gfp inserted before the fkh-9 stop codon. B. An L3 larva of strain UL3146 with gfp inserted after the fkh-9a start codon. C. The head of an L4 larva of strain UL4076 with gfp inserted after the fkh-9b start codon. D. The head of an L4 larva of strain UL4089 with gfp inserted before the fkh-9 stop codon but with exon 2 disrupted to eliminate expression of the reporter arising from fkh-9a and fkh-9b. E. The head of an adult of strain UL3998 with gfp inserted before the fkh-9 stop codon but with exon 1 disrupted to eliminate expression of the reporter arising from fkh-9a. F. An adult of strain UL850 transformed with a plasmid-based reporter gene fusion that would reveal expression for transcript b, but not a or c. A-D. The corresponding DIC photomicrograph is provided beneath the fluorescence micrograph. A-E. captured at 400x, F. from [19] captured at 200x magnification.