Analysis of overlapping and adjacent ORFs affecting EcoRI sensitivity. (A) Example survival assay performed to determine if inactivation of three verified genes (YKL075C, NPL3, and TOP3) that overlap ORFs required for EcoRI resistance also affect resistance to EcoRI. (B) Deletion of two of the seven overlapping, verified genes resulted in strong killing by EcoRI. SS, strongly sensitive; R, resistant. (C) Schematic representations of loci containing two genes required for EcoRI resistance that are adjacent to each other.