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Table 2 Survival of EcoR1 s mutants after exposure to MMS, bleomycin or gamma radiation a

From: A multistep genomic screen identifies new genes required for repair of DNA double-strand breaks in Saccharomyces cerevisiae

Mutant MMS (mM) bleo (μg/ml) Γ (krads) Mutant MMS (mM) bleo (μg/ml) Γ (krads)
  1 2 2 4 30 60   1 2 2 4 30 60
RAD52 group:
rad50 SS SS SS SS SS SS rad55 SS SS R SS SS SS
rad51 SS SS SS SS SS SS rad57 SS SS R SS SS SS
rad52 SS SS SS SS SS SS mre11 SS SS SS SS SS SS
rad54 SS SS SS SS SS SS xrs2 SS SS SS SS SS SS
New EcoRIs mutants:
adk1 R R SS SS R R mrps35 R S R S R S
ado1 S S SS SS S S not5 R R R R R R
akr1 R R R R R S nup8 R S S SS R R
apq13 S SS S SS R R och1 R S S SS R R
arp5 R S R R R S psy1 R R R R R R
atp2 S SS S S R R rad5 SS SS S SS R R
bck1 S SS R R R S rem50 R SS R S R R
bik1 R R R R R R rpb9 R SS S SS S SS
bud19 S S SS SS S SS rpl31a R R R R R R
bud30 R R S S S S rsm7 R R S SS R R
bud32 R R SS SS S SS rtf1 R S R SS S SS
bur2 R S R R R R rvs161 R R R SS R S
cax4 R R SS SS S SS sae2 R S R S R S
ccr4 R R S SS R R sam37 S SS S SS R R
cdc4 SS SS SS SS SS SS sco1 R R R R R R
cgi121 R R S S SS SS sfp1 R R R S R R
cis3 S SS R S R R slm4 S SS S SS R R
cnm67 R S R SS S SS spt10 R S SS SS SS SS
ctf4 SS SS R S R R spt20 R R S SS R R
ctf8 S SS R S R S srv2 R R R R R R
dcc1 S SS R S R R taf14 S SS R S R R
ddc1 R SS R R R R trm9 S S S S R R
eaf1 R S R S S S tsr2 R R R S R S
exo1 R R R R S S ubp8 R R R R R R
gcn5 R SS R S R R ubr1 S S R S SS SS
gnd1 S S R SS S SS ume6 R R R R R S
hsp150 R R R R R R vma S S SS SS S SS
htl1 R S SS SS SS SS vph2 R R SS SS R R
ids2 R R R R R S ybr099c S SS R R R R
img2 R R R S R R ydr417c S SS S SS R R
lip5 R R R S S SS ydr433w R SS SS SS R R
lrp1 R R R S R R ygl218w R R R R R R
lsm7 S S R S R S ylr235c SS SS R S R S
mct1 S SS R S S SS yml009w-b S SS R S R R
mms2 S SS R R R R yml012c-a S SS R S R R
mms22 SS SS R S S S ynr068c SS SS S SS R R
mms4 S SS R R R R        
  1. a Mutants were ranked as resistant (R), sensitive (S), or strongly sensitive (SS). Mutants classified as SS exhibited > 25-fold higher killing than wildtype cells using semi-quantitative dilution pronging assays. Γ, gamma radiation.