A multistep genomic screen identifies new genes required for repair of DNA double-strand breaks in Saccharomyces cerevisiae

Table 5 Physical interactions among proteins required for efficient repair of EcoRI-induced DSBs*

Name	Interacting proteins	Name	Interacting proteins
RAD52 group:
Rad50	Mre11, Xrs2, Dun1	Rad55	Rad51, Rad57, Mec1, Rad53
Rad51	Rad52, Rad54, Rad55, Rad57,		Rtt107, Srs2
	Rad59, Rdh54, Mlh1, Rad23,	Rad57	Rad51, Rad55, Rad24, Rtt107,
	Rfa1, Saw1, Sgs1, Srs2		Srs2
Rad52	Bur2, Rad51, Msh6, Rfa1, Rfa2,	Mre11	Rad50, Xrs2, Dmc1, Dna2,
Rad52	Rfa3, Saw1, Slx5		Dnl4, Msh5, Sgs1, Srs2, Yku80
Rad54	Rad51, Mus81	Xrs2	Rad50, Mre11, Lif1
Non-RAD52 group:
Adk1	Bck1, Sir2	Net1	Cac2, Rad53, Sir2
Akr1	Gcn5, Dun1	Not5	Ccr4
Arp5	Taf14	Npl3	Bur2, Spt5
Bck1	Adk1, Lip5	Rad5	Pol30, Rad18, Rev1, Srs2
Bud32	Cgi121	Rem50	Asf1, Pol30
Bur2	Npl3, Rad52, Rfa1, Rfa2	Rpb9	Spt5, Taf14
Ccr4	Not5, Ubr1	Rpl12b	Rpl31a, Rpl39
Cgi121	Bud32, Srs2	Rpl31a	Rpl12b, Rpl39
Cnm67	Mlp2	Rpl39	Rp12b, Rpl31a
Ctf4	Mms22	Rsm7	Mrps35
Ctf8	Dcc1	Rtf1	Spt5
Dcc1	Ctf8	Rvs161	Gcn5
Ddc1	Mec3, Rad17, Rad52, Rad53, Rev7	Sae2	Sir3, Srs2
Gcn5	Akr1, Mms4, Rvs161, Srv2, Spt20	Sfp1	Asf1
	Ubp8, Rad59	Spt5	Npl3, Rpb9, Rtf1, Taf14
Gnd1	Ubr1	Spt20	Gcn5, Ubp8
Lip5	Bck1	Srv2	Gcn5
Mms2	Pol30	Taf14	Arp5, Rpb9, Spt5, Mus81
Mms4	Gcn5, Mus81, Rad27	Top3	Dna2, Sgs1
Mms22	Ctf4, Mms1	Ubp8	Gcn5, Spt20, Sir3
Mrps35	Rsm7	Ubr1	Ccr4, Gnd1, Rad6, Srs2

* Includes proteins predicted to interact physically plus proteins that are shared components of a multisubunit complex. Underlined names indicate proteins encoded by genes that are required for resistance to EcoRI overexpression. Other interacting proteins have previously been linked to DSB repair and/or damage-inducible checkpoints. Associations were taken from the Saccharomyces Genome Database.

ISSN: 1471-2164