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Figure 3 | BMC Genomics

Figure 3

From: Non-canonical protein-DNA interactions identified by ChIP are not artifacts

Figure 3

ChIP/qPCR of core RNAP (β) for cells grown with or without σ32overexpression. ChIP/qPCR measurement (standard method) of σ32 association with dnaK and four DSTs for cells grown without σ32 overexpression (light gray bars) or with σ32 overexpression (dark gray bars). Occupancy units represent background-subtracted enrichment of target regions relative to a control region. Error bars represent the standard deviation from three independent biological replicates. Significant differences between values for no σ32 overexpression and values for σ32 overexpression are indicated (*p < 0.05, **p < 0.01; one-tailed t-test). Note that the left y-axis is specific for dnaK since these occupancy scores are considerably higher than those for the other regions tested.

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