Figure 3

Workflow of high throughput injection and subsequent analysis. Panel A: from left to right; a zebrafish pair are put together to mate, eggs are collected, eggs are distribute into a 1024 well agarose grid, eggs are injected into the yolk at 2 HPF using the automated microinjection system. Panel B: from left to right; after injection, eggs are collected into Petri dishes and incubated at 28°C for a period of 5 days, COPAS analysis is performed on the S. epidermidis and non-injected embryos at 2, 3, 4 and 5 DPI. Panel C: from left to right; from all groups 20 embryos are snap frozen at 6 HPI, 1, 2, 3, 4 and 5 DPI for RNA isolation, amplification and Cy3 labelling, micro-array analysis against Cy5 labelled common reference and data analysis using Rosetta Resolver. Panel D: from left to right; validation of micro-array data was performed by RNAseq analysis of 4 biological replicas of S. epidermidis infected embryos at 5 DPI.