Figure 2From: Changes in the transcriptome of the malaria parasite Plasmodium falciparumduring the initial phase of transmission from the human to the mosquitoChanges in transcript expression levels during the formation and activation of P. falciparum gametocytes. (A) Diagnostic RT-PCR demonstrated the presence of stage-specific transcript in the asexual blood stages of the gametocyte-less strain F12 (F12 ABS) as well as in immature (stage III/IV) and mature (stage V) non-activated gametocytes (GC) and in gametocytes at 30 min p.a. in vitro. ama-1, marker for ABS; pfccp2, marker for GC. Transcript of gene hdac1 was detected in all stages investigated, while no hdac1-specific PCR product was detected in samples lacking reverse transcriptase (RT). (B) Real-time RT-PCR analysis showed changes of transcript expression of 27 of the SSH-identified genes between ABS, immature and mature non-activated GCs and in GCs at 30 min p.a. in vitro. Transcript expression levels were calculated by the 2-ΔCt method; the threshold cycle number (Ct) was normalized with the Ct of the gene encoding for seryl tRNA synthetase as reference.Back to article page