Table 1 Genes of the Plasmodium falciparum gametocyte transcriptome as identified by SSH (comparing mature non-activated gametocytes and gametocytes at 30 min p.a.)

Signaling

PF3D7_0214600

serine/threonine protein kinase

204.3

PF3D7_1356800

serine/threonine protein kinase ARK3

475.7

PF3D7_0302100

serine/threonine protein kinase PfCLK-4/SRPK1

Phosphorylation of SR splicing factors

157.4

PF3D7_1230900

atypical protein kinase, RIO family

111.5

PF3D7_1238900

calcium/calmodulin-dependent protein kinase, PK2

58.9

PF3D7_0624000

hexokinase, HK

55.3

PF3D7_0515300

phosphatidylinositol 3-kinase, PI3K

255.9

PF3D7_1113100

protein tyrosine phosphatase, PRL

25.1

PF3D7_1403900

phosphatase

35.2

PF3D7_1464600

phosphatase

SP

170.5

PF3D7_0210600

conserved Plasmodium protein

SP, calcium-lipid-binding domain CaLB

53.7

PF3D7_1238100

calcyclin binding protein

Calcyclin-binding domain

266.4

PF3D7_1442200

GTP binding protein

118.7

PF3D7_1358600

zinc finger protein

Zinc-Finger domain

118.3

PF3D7_0927200

zinc finger protein

Zinc-Finger domain

189.7

PF3D7_1004300

zinc finger, C3HC4 type

131.7

PF3D7_1433400

conserved Plasmodium membrane protein

7 TMs, PHD C4HC3 zinc-finger-like motifs

682.5

PF3D7_1463900

conserved Plasmodium membrane protein

SP, 7 TMs, EF hand calcium-binding domain

127.1

PF3D7_1233600

asparagine and aspartate rich protein 1, AARP1

Zinc-finger domain, Ring/U-Box

146.3

PF3D7_0530200

triose phosphate transporter iTPT

SP, 7 TMs

59.8

PF3D7_0303900

conserved Plasmodium protein

SP, phosphatidyl-ethanolamine-binding protein PEBP domain

23.2

PF3D7_0818200

14-3-3 protein

Regulative protein, binds signaling enzymes like kinases or TM receptors

30.2

Cell cycle and gene expression

PF3D7_1445900

DEAD/DEAH box ATP-dependent RNA helicase

60.0

PF3D7_1019000

eukaryotic translation initiation factor subunit eIF2A

280.8

PF3D7_1417200

NOT family protein

Regulation of transcript

519.8

PF3D7_1103800

CCR4-NOT transcription complex subunit 1, NOT1

4 TMs

392.0

PF3D7_0622900

ApiAP2 protein

Transcription factor

237.4

PF3D7_0420300

ApiAP2 protein

Transcription factor

400.2

PF3D7_0615400

ribonuclease

323.2

PF3D7_0811300

CCR4-associated factor 1, CAF1

200.2

PF3D7_1034900

methionine-tRNA ligase

101.2

PF3D7_1469700

conserved Plasmodium protein

Homology to the transcriptional mediator subunit Med6

24.6

PF3D7_0513600

deoxyribodipyrimidine photolyase

129.2

PF3D7_1105500

centrin-4 (CEN4)

20.1

PF3D7_1018500

PHF5-like protein

mRNA splicing factor RDS3 domain

12.5

PF3D7_0827800

SET domain protein SET3

Chromatin regulator, nuclear protein, in male gametocytes

283.6

PF3D7_1115200

SET domain protein, SET7

Chromatin regulator

94.3

PF3D7_1360900

polyadenylate-binding protein

RNA-binding domain

44.8

PF3D7_1011800

QF122 antigen

KH domain type 1, RNA binding

131.6

PF3D7_1347500

DNA/RNA-binding protein Alba 4, ALBA4

42.2

Cytoskeleton, IMC, Motor complex

PF3D7_0422300

alpha-tubulin II

Cytoskeletal element

49.7

PF3D7_1246200

actin I

IMC component

41.2

PF3D7_0503400

actin-depolymerizing factor 1, ADF1

13.7

PF3D7_1020200

kinesin

99.1

PF3D7_0724900

kinesin-like protein

222.6

PF3D7_1246400

myosin A tail domain interacting protein, MTIP

IMC component

23.5

PF3D7_1251200

coronin

Actin-binding protein

69.0

PF3D7_0708000

cytoskeleton-associated protein

Cap-Gly domain

103.6

PF3D7_0515700

glideosome-associated protein 40, GAP40

10 TMs, IMC component

51.8

PF3D7_0918000

glideosome-associated protein 50, GAP50

SP, 2 TMs, IMC component

44.6

PF3D7_1351700

Alveolin, ALV6

IMC component

151.2

Proteostasis (Protein trafficking, stabilization and degradation)

PF3D7_0807500

proteasome subunit alpha type 6

Component oft he proteasome core particle

29.5

PF3D7_1466300

proteasome subunit RPN2

Proteasome non-ATPase regulatory subunit 1

132.8

PF3D7_0815800

vacuolar sorting protein, VPS9

Ortholog of Saccharomyces cerevisae vacuolar sorting protein 9

214.7

PF3D7_1136400

conserved Plasmodium protein

TPR domain, mediates protein interactions and multi-protein complexes

107.1

PF3D7_0215400

conserved Plasmodium protein

WD40-domain protein, assembly and stabilization of MPCs

110.8

PF3D7_0818900

heat shock protein 70, HSP70-1

73.9

PF3D7_0917900

heat shock protein 70, HSP70-2

72.4

PF3D7_0306200

activator of Hsp90 ATPase, AHA1

41.6

PF3D7_0708400

heat shock protein 90, HSP90

86.2

PF3D7_0102200

ring-infected erythrocyte surface antigen, RESA

PHIST and DnaJ domains

126.5

Metabolism, general

mal_mito_2

cytochrome c oxidase subunit I, cox1

SP, 12 TMs, component of the respiratory chain in mitochondria

53.0

PF3D7_1444800

fructose 1,6-biphosphate aldolase

Glycolysis pathway

40.1

PF3D7_0806800

vacuolar proton translocating ATPase subunit A

6 TMs

123.0

PF3D7_0922200

S-adenosylmethionine synthetase, SAMS

44.8

PF3D7_0801900

conserved Plasmodium protein

FAD/NADP-binding domain

552.0

PF3D7_0403200

conserved Plasmodium protein

Possibly acetyl-CoA-synthetase

69.4

PF3D7_0511800

myo-inositol 1-phosphate synthase

69.1

PF3D7_0104400

4-hydroxy-3-methylbut-2-enyl diphosphate reductase, LytB

1 TM, protein export domain, non-mevalonate pathway

62.5

cell surface and PVM

PF3D7_1035300

glutamate-rich protein; GLURP

SP, on parasite surface

141.1

PF3D7_1335300

reticulocyte binding protein 2, RH2b

1 TM, on parasite surface, involved in erythrocyte binding

382.9

PF3D7_1228600

merozoite Surface Protein 9, MSP-9

SP, on merozoite surface, involved in erythrocyte binding

86.6

PF3D7_1028700

merozoite TRAP-like protein, MTRAP

SP, 1 TM, belongs to TRAP family, in micronemes and on merozoite surface

58.1

PF3D7_0731500

erythrocyte binding antigen 175, EBA-175

SP, 1 TM, Duffy binding domain, on merozoite surface

174.6

PF3D7_0102500

erythrocyte binding antigen-181, EBA-181

SP, 2 TMs, Duffy binding domain, on merozoite surface

181.2

PF3D7_1035700

duffy binding-like merozoite surface protein, DBLMSP

SP, Duffy binding domain, SPAM

80.3

PF3D7_0202500

early transcribed membrane protein 2, ETRAMP2

SP, 1 TM, integral PVM protein

11.5

PF3D7_0423700

early transcribed membrane protein 4, ETRAMP4

SP, 2TMs, integral PVM protein

10.2

PF3D7_1033200

early transcribed membrane protein 10.2, ETRAMP 10.2

SP, 2TMs, integral PVM protein

38.9

PF3D7_1103500

CPW-WPC family protein

SP, adhesin, cysteine-rich CPW-WPC domain

67.3

PF3D7_0406200

Pfs16

SP, 2 TMs, integral PVM protein of gametocytes

16.7

PF3D7_0930300

merozoite surface protein 1, MSP1

SP, GPI anchor, EGF domain, on merozoite surface, part of MSP complex

195.7

PF3D7_1218800

conserved Plasmodium protein, PSOP17

SP, lectin domain, laminin G2 domain

39.6

PF3D7_0707300

rhoptry-associated membrane antigen, RAMA

GPI anchor, involved in erythrocyte binding

103.6

PF3D7_1021900

10b antigen

Ankyrin repeat region, PHAX RNA-binding domain, interacts with surface proteins like PfEMP1 (Y2H)

267.4

Others

PF3D7_1103100

60S ribosomal protein P1

13.0

PF3D7_1027800

60S ribosomal protein L3

44.2

PF3D7_1408600

40S ribosomal protein S8e

25.1

PF3D7_1358800

40S ribosomal protein S13

17.3

PF3D7_0206200

metabolite/drug transporter

11 TMs, Major facilitator superfamily

62.7

PF3D7_1207700

blood stage antigen 41–3 precursor

SP

43.4

PF3D7_1457000

signal peptide peptidase, SPP

8 TMs

47.6

PF3D7_0320700

signal peptidase complex subunit 2, SPC2

2 TMs, SPC25 domain

21.0

PF3D7_0207700

serine repeat antigen 4, SERA-4

SP, papain family cysteine proteinase

108.7

PF3D7_1033800

plasmepsin VII

SP, 1 TM, aspartyl protease

52.3

PF3D7_0930000

procollagen lysine 5-dioxygenase

2 TMs

66.8

PF3D7_0817600

conserved Plasmodium protein

MAC/PF domain

112.2

PF3D7_0403800

conserved Plasmodium protein

Alpha/beta-hydrolase

83.4

PF3D7_0709900

conserved Plasmodium membrane protein

4 TMs, alpha/beta-hydrolase

274.0

PF3D7_0311600

conserved Plasmodium protein, unknown function

SP, 1 TM, ribophorin-I domain,putative dolichyl-diphospho-oligosaccharide-protein

84.0

Unknown

PF3D7_0904200

conserved Plasmodium protein

SP, 1 TM

34.0

PF3D7_1024800

conserved Plasmodium protein

SP, 2 TMs

171.1

PF3D7_1239400

conserved Plasmodium protein

SP

22.0

PF3D7_0730800.1

Plasmodium exported protein

SP

32.1

PF3D7_1316700

conserved Plasmodium protein

SP

73.4

PF3D7_0601900

conserved Plasmodium protein

1 TM

14.7

PF3D7_0704100

conserved Plasmodium membrane protein

6 TMs

425.3

PF3D7_1023000

conserved Plasmodium membrane protein

5 TMs

48.9

PF3D7_1439600

conserved Plasmodium protein

1 TM

22.6

PF3D7_1021700

conserved Plasmodium membrane protein

4 TMs

830.2

PF3D7_0417400

conserved Plasmodium protein

2 TMs, interacts with PF3D7_1021700 (Y2H)

822.4

PF3D7_1026600

conserved Plasmodium protein

Interacts with RNA-associated proteins (Y2H)

196.5

PF3D7_1362600

conserved Plasmodium protein

Interacts with splicing factor 3B subunit 2 (Y2H)

32.1

PF3D7_0202400

conserved Plasmodium protein

Pfg27 domain

141.9

PF3D7_1124200

conserved Plasmodium protein

UAS domain

49.3

PF3D7_1452400

conserved Plasmodium protein

107.5

PF3D7_1225600

conserved Plasmodium protein

93.1

PF3D7_1451200

conserved Plasmodium protein

176.2

PF3D7_1438800

conserved Plasmodium protein

84.0

PF3D7_1114600

conserved Plasmodium protein

39.6

PF3D7_1026100

conserved Plasmodium protein

23.9

PF3D7_1230100

conserved Plasmodium protein

61.5

PF3D7_1321000

conserved Plasmodium protein

80.6

PF3D7_0411000

conserved Plasmodium protein

179.7

PF3D7_1126700

conserved Plasmodium protein

110.1

PF3D7_0508900

conserved Plasmodium protein

370.2