MP insert size distribution and library complexity. (a) Insert size distribution of all mate-paired libraries and biological duplicates. Data have been filtered for non-clonal pairs. (b) Complexity of each library is depicted by the number of unique read-pairs versus the number of properly mapped read-pairs. On the x-axis, increasing sequencing depth is represented based on actual sequencing data versus the amount of unique information obtained on the y-axis. A plateau indicates that a library has been sequenced to saturation.