Table 2 Scaffolding value of different paired-read library combinations
From: Improving mammalian genome scaffolding using large insert mate-pair next-generation sequencing
No. of libraries* | Most efficient | Scaffold N50 | Least efficient | Scaffold N50 |
|---|---|---|---|---|
1 | 15 kb | 163,475 | PE | 37,694 |
2 | 5 kb + 25 kb | 522,027 | PE + 3 kb | 46,699 |
2 | 5 kb + 20 kb | 474,308 | PE + 5 kb | 141,403 |
2 | 8 kb + 25 kb | 470,890 | PE + 25 kb | 142,007 |
3 | 5 kb + 20 kb + 25 kb | 834,964 | PE + 3 kb + 5 kb | 158,525 |
3 | 5 kb + 15 kb + 25 kb | 789,954 | PE + 3 kb + 8 kb | 171,253 |
3 | 8 kb + 20 kb + 25 kb | 726,289 | PE + 3 kb + 25 kb | 198,696 |
7 | ALL | 1,287,609 | N/A | N/A |