Figure 3From: Long-range transcriptional regulation by the p110 CUX1 homeodomain protein on theENCODE arrayOverexpression and Knockdown of CUX1 and Expression ProfilingValidation. (A) Hs578t cells were infected with alentiviral vector expressing p110 CUX1 or nothing (vector). RNA andproteins were purified 48 hours post-infection. CUX1 expression wasanalyzed by RT-qPCR and immunoblotting. (B) Hs578t cells wereinfected with a lentiviral vector expressing CUX1 shRNA or a scrambledRNA. RNA and proteins were purified 5 days afterinfection. CUX1 expression was analyzed by and RT-qPCR andimmunoblotting. (C) RNA levels of the indicated genes weremeasured by RT-qPCR in cells treated as in A. Expected up or downindicates regulation that was observed by expression profiling.(D) RNA levels of the indicated genes were measured byRT-qPCR in cells treated as in B. Expected up or down indicatesregulation that was observed by expression profiling. * p<0.05, ***p<0.001 on a Student's T test.Back to article page