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Figure 3 | BMC Genomics

Figure 3

From: Long-range transcriptional regulation by the p110 CUX1 homeodomain protein on theENCODE array

Figure 3

Overexpression and Knockdown of CUX1 and Expression ProfilingValidation. (A) Hs578t cells were infected with alentiviral vector expressing p110 CUX1 or nothing (vector). RNA andproteins were purified 48 hours post-infection. CUX1 expression wasanalyzed by RT-qPCR and immunoblotting. (B) Hs578t cells wereinfected with a lentiviral vector expressing CUX1 shRNA or a scrambledRNA. RNA and proteins were purified 5 days afterinfection. CUX1 expression was analyzed by and RT-qPCR andimmunoblotting. (C) RNA levels of the indicated genes weremeasured by RT-qPCR in cells treated as in A. Expected up or downindicates regulation that was observed by expression profiling.(D) RNA levels of the indicated genes were measured byRT-qPCR in cells treated as in B. Expected up or down indicatesregulation that was observed by expression profiling. * p<0.05, ***p<0.001 on a Student's T test.

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