Transcriptome profile analysis of young floral buds of fertile and sterile plants from the self-pollinated offspring of the hybrid between novel restorer line NR1 and Nsa CMS line in Brassica napus

Table 2 Annotation of distinct Solexa tags against reference sequences of B. rapa and B. oleracea, respectively

	Ste				Fer
Reference sequences	B. oleracea		B. rapa		B. oleracea		B. rapa
Clean distinct tag	242163		242163		253507		253507
Perfect match(Sense)
1 tag->1 gene	67633	27.93%	60153	24.84%	72088	28.44%	64735	25.54%
1 tag->n gene	9238	3.81%	7449	3.08%	9908	3.91%	7975	3.15%
1 bp Mismatch(Sense)
1 tag->1 gene	58766	24.27%	55215	22.80%	58677	23.15%	55444	21.87%
1 tag->n gene	18495	7.64%	16709	6.90%	17955	7.08%	16434	6.48%
Perfect match(Antisense)
1 tag->1 gene	4560	1.88%	4714	1.95%	4400	1.74%	4581	1.81%
1 tag->n gene	935	0.39%	809	0.33%	839	0.33%	728	0.29%
1 bp Mismatch(Antisense)
1 tag->1 gene	1770	0.73%	2395	0.99%	1809	0.71%	2530	1.00%
1 tag->n gene	342	0.14%	323	0.13%	368	0.15%	290	0.11%
Mapping to genome
Perfect match
1 tag->1 position	9375	3.87%	10515	4.34%	9904	3.91%	11025	4.35%
1 tag->n position	933	0.39%	512	0.21%	1045	0.41%	582	0.23%
1 bp Mismatch
1 tag->1 position	10448	4.31%	13951	5.76%	11326	4.47%	14545	5.74%
1 tag->n position	2140	0.88%	1560	0.64%	2327	0.92%	1817	0.72%
Reference genes	45758		41174		45758		41174
Unambiguously matched genes	23909	52.25%	23206	56.36%	25495	55.72%	24819	60.28%
Unknown distinct tag	57528	23.76%	67858	28.02%	62861	24.80%	72821	28.73%

ISSN: 1471-2164