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Table 2 Annotation of distinct Solexa tags against reference sequences of B. rapa and B. oleracea, respectively

From: Transcriptome profile analysis of young floral buds of fertile and sterile plants from the self-pollinated offspring of the hybrid between novel restorer line NR1 and Nsa CMS line in Brassica napus

  Ste Fer
Reference sequences B. oleracea   B. rapa   B. oleracea   B. rapa  
Clean distinct tag 242163   242163   253507   253507  
Perfect match(Sense)
1 tag->1 gene 67633 27.93% 60153 24.84% 72088 28.44% 64735 25.54%
1 tag->n gene 9238 3.81% 7449 3.08% 9908 3.91% 7975 3.15%
1 bp Mismatch(Sense)
1 tag->1 gene 58766 24.27% 55215 22.80% 58677 23.15% 55444 21.87%
1 tag->n gene 18495 7.64% 16709 6.90% 17955 7.08% 16434 6.48%
Perfect match(Antisense)
1 tag->1 gene 4560 1.88% 4714 1.95% 4400 1.74% 4581 1.81%
1 tag->n gene 935 0.39% 809 0.33% 839 0.33% 728 0.29%
1 bp Mismatch(Antisense)
1 tag->1 gene 1770 0.73% 2395 0.99% 1809 0.71% 2530 1.00%
1 tag->n gene 342 0.14% 323 0.13% 368 0.15% 290 0.11%
Mapping to genome
Perfect match
1 tag->1 position 9375 3.87% 10515 4.34% 9904 3.91% 11025 4.35%
1 tag->n position 933 0.39% 512 0.21% 1045 0.41% 582 0.23%
1 bp Mismatch
1 tag->1 position 10448 4.31% 13951 5.76% 11326 4.47% 14545 5.74%
1 tag->n position 2140 0.88% 1560 0.64% 2327 0.92% 1817 0.72%
Reference genes 45758   41174   45758   41174  
Unambiguously matched genes 23909 52.25% 23206 56.36% 25495 55.72% 24819 60.28%
Unknown distinct tag 57528 23.76% 67858 28.02% 62861 24.80% 72821 28.73%